Bayer MaterialScience LLC, Pittsburgh, PA, USA.
Acta Biomater. 2012 May;8(5):1869-80. doi: 10.1016/j.actbio.2012.01.032. Epub 2012 Feb 4.
Biofilm-associated infections are a major complication of implanted and indwelling medical devices like urological and venous catheters. They commonly persist even in the presence of an oral or intravenous antibiotic regimen, often resulting in chronic illness. We have developed a new approach to inhibiting biofilm growth on synthetic materials through controlled release of salicylic acid from a polymeric coating. Herein we report the synthesis and testing of a ultraviolet-cured polyurethane acrylate polymer composed, in part, of salicyl acrylate, which hydrolyzes upon exposure to aqueous conditions, releasing salicylic acid while leaving the polymer backbone intact. The salicylic acid release rate was tuned by adjusting the polymer composition. Anti-biofilm performance of the coatings was assessed under several biofilm forming conditions using a novel combination of the MBEC Assay™ biofilm multi-peg growth system and bioluminescence monitoring for live cell quantification. Films of the salicylic acid-releasing polymers were found to inhibit biofilm formation, as shown by bioluminescent and GFP reporter strains of Pseudomonas aeruginosa and Escherichia coli. Urinary catheters coated on their inner lumens with the salicylic acid-releasing polymer significantly reduced biofilm formation by E. coli for up to 5 days under conditions that simulated physiological urine flow.
生物膜相关感染是植入和留置医疗设备（如泌尿科和静脉导管）的主要并发症。即使在口服或静脉内抗生素治疗方案存在的情况下，它们通常也会持续存在，常常导致慢性疾病。我们开发了一种通过从聚合物涂层中控制释放水杨酸来抑制合成材料上生物膜生长的新方法。在这里，我们报告了一种紫外光固化的聚氨酯丙烯酸酯聚合物的合成和测试，该聚合物部分由水杨酸丙烯酯组成，在暴露于水条件下会水解，释放水杨酸，同时保持聚合物主链完整。通过调整聚合物组成来调整水杨酸的释放速率。在几种生物膜形成条件下，使用新型 MBEC 测定法™生物膜多钉生长系统和生物发光监测活细胞定量的组合，评估了涂层的抗生物膜性能。结果表明，释放水杨酸的聚合物薄膜可以抑制生物膜的形成，这是通过绿脓假单胞菌和大肠杆菌的生物发光和 GFP 报告株来证明的。在模拟生理尿液流动条件下，将水杨酸释放聚合物涂覆在内腔的导尿管可显著减少大肠杆菌生物膜的形成，长达 5 天。
