Malbica J O, Monson K R
J Pharm Sci. 1975 Dec;64(12):1992-4. doi: 10.1002/jps.2600641219.
A method is described for the GLC determination of atenolol BP in plasma and urine. Extraction is accomplished under dehydrating conditions, and interfering impurities are removed by using an acidified cyclohexane-isopropanol mixture (2:1) and charcoal-treated paper disks. The drug thus isolated appears to react more efficiently with heptafluorobutyric anhydride, increasing the sensitivity of GLC electron-capture analysis. Concentrations as low as 0.02 mug/ml were measured using 0.5-ml aliquots of plasma or 0.1 ml of urine. Amino alcohols such as atenolol may form hydrates or alcoholates, precluding complete derivatization with heptafluorobutyric anhydride.
本文描述了一种用气相色谱法测定血浆和尿液中英国药典规定的阿替洛尔的方法。提取在脱水条件下完成，通过使用酸化的环己烷 - 异丙醇混合物（2:1）和经活性炭处理的纸盘去除干扰杂质。如此分离出的药物似乎能更有效地与七氟丁酸酐反应，提高了气相色谱电子捕获分析的灵敏度。使用0.5毫升血浆等分试样或0.1毫升尿液可测定低至0.02微克/毫升的浓度。像阿替洛尔这样的氨基醇可能会形成水合物或醇化物，从而妨碍与七氟丁酸酐的完全衍生化。
