Scales B, Copsey P B
J Pharm Pharmacol. 1975 Jun;27(6):430-3. doi: 10.1111/j.2042-7158.1975.tb09473.x.
Methods are described for the gas chromatographic determination of the B-adrenoceptor blocking agent atenolol (ICI 66,082 Tenormin) in whole blood, serum, tissue and urine. The method involves the extraction of the free base from the prepared biological sample into an organic solvent mixture. Further purification is done by backextracting the base into dilute acid from which, after basification it is extracted again into organic solvent. Any atenolol in the residue remaining after solvent evaporation is converted into a heptafluorobutyrate derivative ready for gas chromatography. Use of an electron-capture detector enables concentrations as low as 0.01 mug ml-minus 1 body fluid and 0.04 mug ml-minus 1 tissue to be measured.
本文描述了气相色谱法测定全血、血清、组织和尿液中β-肾上腺素受体阻滞剂阿替洛尔（ICI 66,082 天诺敏）的方法。该方法包括将制备好的生物样品中的游离碱萃取到有机溶剂混合物中。进一步纯化是通过将碱反萃取到稀酸中，碱化后再将其萃取回有机溶剂中。溶剂蒸发后残留的任何阿替洛尔都转化为七氟丁酸酯衍生物，以便进行气相色谱分析。使用电子捕获检测器能够测量低至0.01μg/ml的体液浓度和0.04μg/ml的组织浓度。
