Ashby Michael C, Ibaraki Kyoko, Henley Jeremy M
MRC Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.
Trends Neurosci. 2004 May;27(5):257-61. doi: 10.1016/j.tins.2004.03.010.
Green fluorescent protein (GFP) and mutated GFP variants have proved to be immensely powerful tools that have had a profound impact on research in biological sciences. This review considers the development, use and future implications of pH-dependent GFP variants (e.g. pHluorins). These proteins hold considerable promise for the relatively non-invasive monitoring of events such as exocytosis, endocytosis and protein surface expression in living neurons with high spatial and temporal resolution.
绿色荧光蛋白（GFP）及其突变体已被证明是极为强大的工具，对生物科学研究产生了深远影响。本综述探讨了pH依赖型GFP变体（如pHluorins）的发展、应用及未来意义。这些蛋白质在以高时空分辨率对活神经元中的胞吐作用、内吞作用及蛋白质表面表达等事件进行相对非侵入性监测方面具有巨大潜力。
