González V, Padilla E, Giménez M, Vilaplana C, Pérez A, Fernández G, Quesada M D, Pallarés M A, Ausina V
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera. del Canyet s/n, Badalona, 08916 Barcelona, Spain.
Eur J Clin Microbiol Infect Dis. 2004 May;23(5):396-8. doi: 10.1007/s10096-004-1112-x. Epub 2004 Apr 27.
In the study presented here, the performance of the S. aureus PNA FISH assay was evaluated using 285 blood cultures (from 104 patients) that had gram-positive cocci resembling staphylococci on Gram stain. The new molecular test is based on a fluorescence in situ hybridization assay using peptide nucleic acid probes targeting Staphylococcus aureus 16S rRNA and is designed for the rapid identification of Staphylococcus aureus directly from positive blood cultures. The sensitivity, specificity, and positive and negative predictive values of the S. aureus PNA FISH for the rapid identification of Staphylococcus aureus directly from positive blood culture bottles were 100, 99.4, 99.2 and 100%, respectively.
在本研究中，使用285份血培养标本（来自104名患者）对金黄色葡萄球菌肽核酸荧光原位杂交检测法（S. aureus PNA FISH检测法）的性能进行了评估，这些血培养标本经革兰氏染色显示有类似葡萄球菌的革兰氏阳性球菌。这项新的分子检测基于一种荧光原位杂交检测法，使用靶向金黄色葡萄球菌16S rRNA的肽核酸探针，旨在直接从阳性血培养标本中快速鉴定金黄色葡萄球菌。金黄色葡萄球菌PNA FISH检测法直接从阳性血培养瓶中快速鉴定金黄色葡萄球菌的敏感性、特异性、阳性预测值和阴性预测值分别为100%、99.4%、99.2%和100%。
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