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通过使用肽核酸探针的荧光原位杂交和流式细胞术相结合的方法快速鉴定血培养中的金黄色葡萄球菌。

Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry.

作者信息

Hartmann Hanna, Stender Henrik, Schäfer Andrea, Autenrieth Ingo B, Kempf Volkhard A J

机构信息

Institut für Medizinische Mikrobiologie und Hygiene, Tübingen, Germany.

出版信息

J Clin Microbiol. 2005 Sep;43(9):4855-7. doi: 10.1128/JCM.43.9.4855-4857.2005.

DOI:10.1128/JCM.43.9.4855-4857.2005
PMID:16145158
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1234125/
Abstract

Fluorescence in situ hybridization (FISH) using peptide nucleic acid probes (PNAs) allows the identification of Staphylococcus aureus from human blood culture samples. We present data revealing that the combination of PNA FISH and flow cytometry is a possible approach for the noncultural identification of staphylococci in blood cultures.

摘要

使用肽核酸探针（PNA）的荧光原位杂交（FISH）技术可用于从人血培养样本中鉴定金黄色葡萄球菌。我们提供的数据表明，PNA FISH与流式细胞术相结合是一种对血培养中的葡萄球菌进行非培养鉴定的可行方法。

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本文引用的文献

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Int J Med Microbiol. 2005 Apr;295(1):47-55. doi: 10.1016/j.ijmm.2004.12.006.
2
Septic shock.感染性休克
Lancet. 2005;365(9453):63-78. doi: 10.1016/S0140-6736(04)17667-8.
3
Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH.使用金黄色葡萄球菌肽核酸荧光原位杂交技术快速诊断金黄色葡萄球菌菌血症
Eur J Clin Microbiol Infect Dis. 2004 May;23(5):396-8. doi: 10.1007/s10096-004-1112-x. Epub 2004 Apr 27.
4
The epidemiology of sepsis in the United States from 1979 through 2000.1979年至2000年美国败血症的流行病学情况。
N Engl J Med. 2003 Apr 17;348(16):1546-54. doi: 10.1056/NEJMoa022139.
5
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J Clin Microbiol. 2003 Feb;41(2):889-91. doi: 10.1128/JCM.41.2.889-891.2003.
6
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7
An introduction to peptide nucleic acid.肽核酸简介。
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8
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9
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10
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