Mottla G L, Adelman M R, Hall J L, Gindoff P R, Stillman R J, Johnson K E
Department of Obstetrics and Gynecology, George Washington University School of Medicine and Health Sciences, Washington, DC 20037, USA.
Hum Reprod. 1995 Feb;10(2):384-91. doi: 10.1093/oxfordjournals.humrep.a135949.
We injected a fluorescent lineage tracer (Texas Red-lysine-dextran) into individual blastomeres of donated human diploid 2- to 8-cell pre-embryos and cultured them to blastocysts. Once pre-embryos reached the expanded blastocyst stage, they were fixed and examined in a scanning confocal microscope to identify the location of fluorescent tracer. In successfully injected pre-embryos that developed to expanded blastocysts, we found that randomly injected blastomeres formed both trophectoderm (TE) and inner cell mass (ICM). More labelled progeny were found in TE than in ICM. Our results show that individual early blastomeres are not yet committed to form either TE or ICM but instead can form both rudiments.
我们将一种荧光谱系示踪剂（德克萨斯红赖氨酸葡聚糖）注射到捐赠的人类二倍体2至8细胞期胚胎的单个卵裂球中，并将它们培养至囊胚。一旦胚胎达到扩张囊胚阶段，就将它们固定并在扫描共聚焦显微镜下检查，以确定荧光示踪剂的位置。在成功注射并发育到扩张囊胚的胚胎中，我们发现随机注射的卵裂球形成了滋养外胚层（TE）和内细胞团（ICM）。在TE中发现的标记后代比在ICM中更多。我们的结果表明，单个早期卵裂球尚未定向形成TE或ICM，而是可以形成两者的雏形。
