Brito Maria Edileuza Felinto de, Almeida Ericka Lima, Medeiros Angela Cristina Rapela, Werkhäuser Roberto Pereira, Alexandre Joanna Lucia de Almeida, Sá Bruna Santos Lima Figueiredo, Rodrigues Eduardo Henrique Gomes, Brandão-Filho Sinval Pinto
Fundação Oswaldo Cruz-Fiocruz, Instituto Aggeu Magalhães, Departamento de Imunologia, Recife, PE, Brasil.
Universidade de Pernambuco, Recife, PE, Brasil.
Mem Inst Oswaldo Cruz. 2018 Feb 5;113(4):e170250. doi: 10.1590/0074-02760170250.
Several studies have described the use of non-invasive collection methods, mostly based on the detection of parasite DNA, for diagnosis. However, no Leishmania specimens have been isolated from saliva. Here, we report the first isolation of Leishmania braziliensis from the saliva of humans with cutaneous leishmaniasis but without lesions on their mucosa. The isolates were obtained from salivary fluid inoculated in hamsters and were tested by multilocus enzyme electrophoresis. Seven samples from 43 patients suspected of having the disease were identified for in vivo culture. These findings suggest that saliva is a clinical sample that allows the isolation of Leishmania sp.
多项研究描述了使用主要基于寄生虫DNA检测的非侵入性采集方法进行诊断。然而，尚未从唾液中分离出利什曼原虫标本。在此，我们报告首次从患有皮肤利什曼病但黏膜无病变的人类唾液中分离出巴西利什曼原虫。分离株是从接种到仓鼠体内的唾液中获得的，并通过多位点酶电泳进行检测。对43名疑似患有该病的患者的7份样本进行了体内培养鉴定。这些发现表明唾液是一种能够分离出利什曼原虫属的临床样本。
