Böckers Tobias M, Segger-Junius Mailin, Iglauer Peter, Bockmann Jürgen, Gundelfinger Eckart D, Kreutz Michael R, Richter Dietmar, Kindler Stefan, Kreienkamp Hans-Jürgen
Institut für Anatomie Westfälishe Wilhelms-Universität-Münster, Germany.
Mol Cell Neurosci. 2004 May;26(1):182-90. doi: 10.1016/j.mcn.2004.01.009.
Shank proteins are scaffolding proteins in the postsynaptic density of excitatory synapses in the mammalian brain. In situ hybridization revealed that Shank1/SSTRIP and Shank2/ProSAP1 mRNAs are widely expressed early in postnatal brain development whereas Shank3/ProSAP2 expression increases during postnatal development especially in the cerebellum and thalamus. Shank1 and Shank3 (but not Shank2) mRNAs are present in the molecular layers of the hippocampus, consistent with a dendritic transcript localization. Shank1 and Shank2 transcripts are detectable in the dendritic fields of Purkinje cells, whereas Shank3 mRNA is restricted to cerebellar granule cells. The appearance of dendritic Shank mRNAs in cerebellar Purkinje cells coincides with the onset of dendrite formation. Expression of reporter transcripts in hippocampal neurons identifies a 200-nucleotide dendritic targeting element (DTE) in the Shank1 mRNA. The widespread presence of Shank mRNAs in dendrites suggests a role for local synthesis of Shanks in response to stimuli that induce alterations in synaptic morphology.
支架蛋白是哺乳动物大脑中兴奋性突触后致密部的支架蛋白。原位杂交显示,支架蛋白1/ SSTRIP和支架蛋白2/ProSAP1信使核糖核酸在出生后大脑发育早期广泛表达,而支架蛋白3/ProSAP2的表达在出生后发育过程中增加,尤其是在小脑和丘脑。支架蛋白1和支架蛋白3(而非支架蛋白2)的信使核糖核酸存在于海马体的分子层中,这与树突状转录本的定位一致。在浦肯野细胞的树突区域可检测到支架蛋白1和支架蛋白2的转录本,而支架蛋白3信使核糖核酸则局限于小脑颗粒细胞。小脑浦肯野细胞中树突状支架蛋白信使核糖核酸的出现与树突形成的开始相吻合。在海马神经元中报告转录本的表达确定了支架蛋白1信使核糖核酸中的一个200个核苷酸的树突靶向元件(DTE)。树突中广泛存在的支架蛋白信使核糖核酸表明,支架蛋白的局部合成在响应诱导突触形态改变的刺激中发挥作用。