Luan Xin-jun, Song Zheng-hua, Xiao Zhen
Department of Chemistry, Northwest University, Xi'an 710069, China.
Yao Xue Xue Bao. 2004 Jan;39(1):64-7.
To establish a new and simple flow injection method for the rapid determination of acetylspiramycin (ASPM).
ASPM was determined by chemiluminescence (CL) method combined with flow injection (FI) technology, which was based on the inhibitive effect of ASPM on the chemiluminescence reaction of the luminol-K3Fe (CN)6 system.
The decrease of chemiluminescence intensity was proportional to the logarithm of ASPM concentration (0.1-100) microgram.L-1, the detection limit was 40 ng.L-1 (3 sigma). The whole process, including sampling and washing, could be completed in 0.5 min with a RSD less than 3.0% (n = 5).
The FI-CL method is of both high sensitivity and good selectivity giving a throughput of 120 h-1. The proposed method was applied successfully to the determination of ASPM in pharmaceutical preparations and human urine without any pre-treatment. It was found that the ASPM concentration reached its maximum after being orally administrated for two hours.
建立一种新的、简单的流动注射法快速测定乙酰螺旋霉素（ASPM）。
基于ASPM对鲁米诺-K3Fe(CN)6体系化学发光反应的抑制作用，采用化学发光（CL）法结合流动注射（FI）技术测定ASPM。
化学发光强度的降低与ASPM浓度（0.1 - 100）μg·L-1的对数成正比，检测限为40 ng·L-1（3σ）。整个过程，包括进样和清洗，可在0.5分钟内完成，相对标准偏差小于3.0%（n = 5）。
流动注射-化学发光法灵敏度高、选择性好，通量为120 h-1。该方法无需任何预处理即可成功应用于药物制剂和人尿中ASPM的测定。结果发现，口服ASPM两小时后其浓度达到最大值。
Zotero 插件