Shao Xiaodong, Xie Xiaofeng, Liu Yanhong, Song Zhenghua
Department of Chemistry, Northwest University, Xi'an 710069, China.
J Pharm Biomed Anal. 2006 May 3;41(2):667-70. doi: 10.1016/j.jpba.2005.12.027. Epub 2006 Jan 24.
A sensitive chemiluminescence method, based on the inhibitory effect of clindamycin on the chemiluminescence reaction between luminol and myoglobin in a flow-injection system, is proposed for the determination of clindamycin. The decrement of chemiluminescence intensity is linear with the logarithm of clindamycin concentration over the range from 0.1 to 70.0 ng ml-1 (r2=0.9995), with the detection limit of 0.03 ng ml-1 (3sigma). At a flow rate of 2.0 ml min-1, a complete analytical process could be performed within 0.5 min, including sampling and washing, with a relative standard deviation of less than 3.0% (n=5). The proposed procedure was applied successfully to the determination of clindamycin in capsules without any pretreatment process.
基于克林霉素对流动注射体系中鲁米诺与肌红蛋白之间化学发光反应的抑制作用，提出了一种灵敏的化学发光法用于测定克林霉素。化学发光强度的降低与克林霉素浓度的对数在0.1至70.0 ng ml-1范围内呈线性关系（r2 = 0.9995），检测限为0.03 ng ml-1（3σ）。在流速为2.0 ml min-1时，包括进样和清洗在内的完整分析过程可在0.5分钟内完成，相对标准偏差小于3.0%（n = 5）。所提出的方法无需任何预处理过程即可成功应用于胶囊中克林霉素的测定。
