人白细胞介素-10在植物中的亚细胞定位

Subcellular targeting of human interleukin-10 in plants.

作者信息

Menassa Rima, Kennette Wendy, Nguyen Vi, Rymerson Robert, Jevnikar Anthony, Brandle Jim

机构信息

Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, Ont., Canada N5V 4T3.

出版信息

J Biotechnol. 2004 Mar 4;108(2):179-83. doi: 10.1016/j.jbiotec.2003.11.009.

DOI:10.1016/j.jbiotec.2003.11.009

PMID:15129727

Abstract

The utility of plants for the production of a wide range of recombinant proteins is now clearly established. However, the challenge remains to produce these proteins at sufficient concentrations for extraction to be economically feasible. In this paper, we have investigated the ability of plant cells to accumulate the human interleukin-10 (IL-10) protein targeted to chloroplasts and mitochondria. We found that IL-10 accumulates in chloroplasts only if a 6 x His tag is added at the C-terminus of the protein. The hexapeptide may provide protection from degradation. Conversely, the IL-10 protein does not accumulate in mitochondria. Analysis of the chloroplast-targeted IL-10 protein revealed only monomeric IL-10 and limited biological activity in in vitro cell assays.

摘要

植物用于生产多种重组蛋白的效用现已得到明确证实。然而，挑战依然存在，即要以足够的浓度生产这些蛋白，以使提取在经济上可行。在本文中，我们研究了植物细胞积累靶向叶绿体和线粒体的人白细胞介素-10（IL-10）蛋白的能力。我们发现，只有在该蛋白的C末端添加6×组氨酸标签时，IL-10才会在叶绿体中积累。该六肽可能提供抗降解保护。相反，IL-10蛋白在线粒体中不积累。对靶向叶绿体的IL-10蛋白的分析显示，在体外细胞试验中仅存在单体IL-10且生物活性有限。