用于组织电穿孔成像的电阻抗断层成像技术。

Electrical impedance tomography for imaging tissue electroporation.

作者信息

Davalos Rafael V, Otten David M, Mir Lluis M, Rubinsky Boris

机构信息

Biomedical Engineering Laboratory, Department of Mechanical Engineering, 6178 Etcheverry Hall, University of California at Berkeley, Berkeley, CA 94720-1740, USA.

出版信息

IEEE Trans Biomed Eng. 2004 May;51(5):761-7. doi: 10.1109/TBME.2004.824148.

DOI:10.1109/TBME.2004.824148

PMID:15132502

Abstract

Electroporation is a method to introduce molecules, such as gene constructs or small drugs, into cells by temporarily permeating the cell membrane with electric pulses. In molecular medicine and biotechnology, tissue electroporation is performed with electrodes placed in the target area of the body. Currently, tissue electroporation, as with all other methods of molecular medicine, is performed without real-time control or near-term information regarding the extent and degree of electroporation. This paper expands the work from our previous study by implementing new ex vivo experimental data with "front-tracking" analysis for the image reconstruction algorithm. The experimental data is incorporated into numerical simulations of electroporation procedures and images are generated using the new reconstruction algorithm to demonstrate that electrical impedance tomography (EIT) can produce an image of the electroporated area. Combining EIT with electroporation could become an important biotechnological and medical technique to introduce therapeutic molecules into cells in tissue at predetermined areas of the body.

摘要

电穿孔是一种通过电脉冲暂时穿透细胞膜将分子（如基因构建体或小分子药物）导入细胞的方法。在分子医学和生物技术中，组织电穿孔是通过将电极置于身体的目标区域来进行的。目前，与所有其他分子医学方法一样，组织电穿孔在没有关于电穿孔程度和范围的实时控制或近期信息的情况下进行。本文通过对图像重建算法进行“前沿追踪”分析，采用新的离体实验数据，扩展了我们之前研究的工作。将实验数据纳入电穿孔过程的数值模拟，并使用新的重建算法生成图像，以证明电阻抗断层扫描（EIT）可以生成电穿孔区域的图像。将EIT与电穿孔相结合可能成为一种重要的生物技术和医学技术，用于在身体的预定区域将治疗性分子导入组织中的细胞。