Smoyer Laura K, Dorer Douglas R, Nickerson Kenneth W, Christensen Alan C
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118, USA.
Genet Res. 2003 Dec;82(3):163-70. doi: 10.1017/s001667230300644x.
The Triplo-lethal locus (Tpl) of Drosophila is both triplo-lethal and haploinsufficient, but the function of the locus is unknown. We have examined Tpl-aneuploid embryos and find that, in both trisomics and monosomics, the midgut shows extensive cell death and the tracheae are abnormal. Shortly thereafter, all tissues die. PCR-based genotyping of individual embryos and larvae show that this phenotype occurs in the trisomics after hatching and in the monosomics before hatching. Weak alleles of the interacting gene Su(Tpl) delay the death of Tpl trisomics, but they still show the same tracheal and midgut phenotypes before dying. Hyperoxia (45% oxygen) partially suppresses the phenotype of Tpl aneuploids, even though the use of a hypoxia reporter strain shows that dying Tpl aneuploids are not hypoxic. This is the first report of a phenotype associated with the Tpl locus and the first report of an environmental condition that suppresses the phenotype.
果蝇的三倍体致死基因座（Tpl）既是三倍体致死的，又是单倍体不足的，但该基因座的功能尚不清楚。我们检查了Tpl非整倍体胚胎，发现无论是三体还是单体，中肠都出现广泛的细胞死亡，气管也不正常。此后不久，所有组织都会死亡。对单个胚胎和幼虫进行基于PCR的基因分型表明，这种表型在孵化后的三体中出现，在孵化前的单体中出现。相互作用基因Su(Tpl)的弱等位基因会延迟Tpl三体的死亡，但它们在死亡前仍表现出相同的气管和中肠表型。高氧（45%氧气）部分抑制了Tpl非整倍体的表型，尽管使用缺氧报告菌株表明即将死亡的Tpl非整倍体并不缺氧。这是与Tpl基因座相关表型的首次报告，也是抑制该表型的环境条件的首次报告。
