通过增加一个不连锁基因座的剂量来抑制黑腹果蝇的致死三体表型。
Suppression of a lethal trisomic phenotype in Drosophila melanogaster by increased dosage of an unlinked locus.
作者信息
Dorer D R, Cadden M A, Gordesky-Gold B, Harries G, Christensen A C
机构信息
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-5541.
出版信息
Genetics. 1993 May;134(1):243-9. doi: 10.1093/genetics/134.1.243.
One of the most extreme examples of gene dosage sensitivity is the Triplo-lethal locus (Tpl) on the third chromosome of Drosophila melanogaster, which is lethal when present in either one or three copies. Increased dosage of an unlinked locus, Isis, suppresses the triplo-lethal phenotype of Tpl, but not the haplo-lethal phenotype. We have mapped Isis to the X chromosome region 7E3-8A5, and shown that the suppression is a gene dosage effect. Altered dosage of Isis in the presence of two copies of Tpl has no obvious effects. By examining the interactions between Isis dosage and Tpl we suggest that Isis does not directly repress Tpl expression, but acts downstream on the triplo-lethal phenotype of Tpl.
基因剂量敏感性最极端的例子之一是黑腹果蝇第三条染色体上的三倍体致死位点（Tpl），该位点在单拷贝或三拷贝时均具有致死性。一个不连锁的位点Isis剂量增加可抑制Tpl的三倍体致死表型，但不能抑制单倍体致死表型。我们已将Isis定位到X染色体区域7E3 - 8A5，并表明这种抑制是一种基因剂量效应。在存在两份Tpl的情况下，改变Isis的剂量没有明显影响。通过研究Isis剂量与Tpl之间的相互作用，我们认为Isis并不直接抑制Tpl的表达，而是在Tpl的三倍体致死表型的下游起作用。
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