Schwender Jörg, Ohlrogge John, Shachar-Hill Yair
Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824-1312, USA.
Curr Opin Plant Biol. 2004 Jun;7(3):309-17. doi: 10.1016/j.pbi.2004.03.016.
The revolutionary growth in our ability to identify the 'parts list' of cellular infrastructure in plants in detail, and to alter it with precision, challenges us to develop methods to quantify how these parts function. For components of metabolism, this means mapping fluxes at the level of metabolic networks. Advances in experimental, analytical and software tools for metabolic flux analysis now allow maps of the fluxes through central metabolism to be obtained from the results of stable-isotope-labeling experiments. Such maps have led to notable successes in understanding and engineering metabolic function in microorganisms. Recent studies in plants are giving insight into particular fluxes, such as those of the pentose phosphate pathway, and into general phenomena, such as substrate- or futile-cycles and compartmentation. The importance of experimental design and statistical analysis have been illustrated, and analyses of fluxes in heterotrophic plant tissues have been carried out recently.
我们详细识别植物细胞基础设施“部件清单”并精确改变它的能力取得了革命性进展，这促使我们开发方法来量化这些部件的功能。对于代谢成分而言，这意味着在代谢网络层面绘制通量图谱。代谢通量分析的实验、分析和软件工具的进步，现在使得能够从稳定同位素标记实验的结果中获得通过中心代谢的通量图谱。这样的图谱在理解和改造微生物代谢功能方面取得了显著成功。最近在植物方面的研究正在深入了解特定的通量，如磷酸戊糖途径的通量，以及一般现象，如底物循环或无效循环和区室化。实验设计和统计分析的重要性已得到说明，并且最近对异养植物组织中的通量进行了分析。
