一种类弗林蛋白酶对鼠冠状病毒刺突蛋白的切割抑制作用影响细胞间融合，但不影响病毒与细胞的融合。
Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion.
作者信息
de Haan Cornelis A M, Stadler Konrad, Godeke Gert-Jan, Bosch Berend Jan, Rottier Peter J M
机构信息
Virology Division, Department of Infectious Diseases & Immunology, Yalelaan 1, 3584CL Utrecht, The Netherlands.
出版信息
J Virol. 2004 Jun;78(11):6048-54. doi: 10.1128/JVI.78.11.6048-6054.2004.
Abstract
Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.
摘要
一种肽类弗林蛋白酶抑制剂以浓度依赖的方式阻断了小鼠肝炎病毒A59株刺突蛋白的切割，这表明弗林蛋白酶或类弗林蛋白酶负责这一过程。虽然阻止刺突蛋白切割明显影响细胞-细胞融合，但病毒-细胞融合不受影响，这表明这些事件有不同的要求。
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本文引用的文献
1
Coronaviruses as vectors: position dependence of foreign gene expression.
J Virol. 2003 Nov;77(21):11312-23. doi: 10.1128/jvi.77.21.11312-11323.2003.
2
The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex.
J Virol. 2003 Aug;77(16):8801-11. doi: 10.1128/jvi.77.16.8801-8811.2003.
3
Conformational changes in the spike glycoprotein of murine coronavirus are induced at 37 degrees C either by soluble murine CEACAM1 receptors or by pH 8.
J Virol. 2003 Jan;77(2):830-40. doi: 10.1128/jvi.77.2.830-840.2003.
4
The virulence of mouse hepatitis virus strain A59 is not dependent on efficient spike protein cleavage and cell-to-cell fusion.
J Neurovirol. 2002 Oct;8(5):400-10. doi: 10.1080/13550280260422703.
5
Infectious entry by amphotropic as well as ecotropic murine leukemia viruses occurs through an endocytic pathway.
J Virol. 2001 Jun;75(11):5018-26. doi: 10.1128/JVI.75.11.5018-5026.2001.
6
Assembly of spikes into coronavirus particles is mediated by the carboxy-terminal domain of the spike protein.
J Virol. 2000 Feb;74(3):1566-71. doi: 10.1128/jvi.74.3.1566-1571.2000.
7
A 12-amino acid stretch in the hypervariable region of the spike protein S1 subunit is critical for cell fusion activity of mouse hepatitis virus.
J Biol Chem. 1999 Sep 10;274(37):26085-90. doi: 10.1074/jbc.274.37.26085.
8
Quantitative characterization of furin specificity. Energetics of substrate discrimination using an internally consistent set of hexapeptidyl methylcoumarinamides.
J Biol Chem. 1999 Aug 13;274(33):23229-34. doi: 10.1074/jbc.274.33.23229.
9
Effect of bafilomycin A1 and nocodazole on endocytic transport in HeLa cells: implications for viral uncoating and infection.
J Virol. 1998 Dec;72(12):9645-55. doi: 10.1128/JVI.72.12.9645-9655.1998.
10
The function of the spike protein of mouse hepatitis virus strain A59 can be studied on virus-like particles: cleavage is not required for infectivity.
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