Martínez-Cánovas M José, Quesada Emilia, Llamas Inmaculada, Béjar Victoria
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
Int J Syst Evol Microbiol. 2004 May;54(Pt 3):733-737. doi: 10.1099/ijs.0.02942-0.
Halomonas ventosae sp, nov. includes three moderately halophilic, exopolysaccharide-producing strains isolated from saline soils in Jaén (south-eastern Spain). These strains can grow anaerobically using either nitrate or nitrite as terminal electron acceptor and hydrolyse both tyrosine and phenylalanine. Their G+C content varies between 72.6 and 74.3 mol%. The affiliation of the isolates with the genus Halomonas was confirmed by 16S rRNA gene sequence comparison. DNA-DNA hybridization shows 70.4-82.7 % relatedness among the three strains. Nevertheless, their relatedness is less than 43 % compared to related reference strains. The proposed type strain for Halomonas ventosae is strain Al12(T) (=CECT 5797(T)=DSM 15911(T)). It grows best at 8 % (w/v) sea salts and requires the presence of Na(+). Its major fatty acids are 18 : 1 omega7c, 16 : 0, 16 : 1 omega7c, and 15 : 0 iso 2-OH. The predominant respiratory lipoquinone found in strain Al12(T) is ubiquinone with nine isoprene units (Q-9).
嗜盐嗜风单胞菌新种。包括从西班牙东南部哈恩的盐渍土中分离出的三株中度嗜盐、产胞外多糖的菌株。这些菌株可以利用硝酸盐或亚硝酸盐作为末端电子受体进行厌氧生长,并能水解酪氨酸和苯丙氨酸。它们的G+C含量在72.6至74.3 mol%之间。通过16S rRNA基因序列比较证实了分离菌株与嗜盐单胞菌属的亲缘关系。DNA-DNA杂交显示这三株菌株之间的相关性为70.4-82.7%。然而,与相关参考菌株相比,它们的相关性小于43%。嗜盐嗜风单胞菌的模式菌株为Al12(T)菌株(=CECT 5797(T)=DSM 15911(T))。它在8%(w/v)海盐浓度下生长最佳,且需要有Na(+)存在。其主要脂肪酸为18 : 1 ω7c、16 : 0、16 : 1 ω7c和15 : 0 iso 2-OH。在Al12(T)菌株中发现的主要呼吸性脂醌是含有九个异戊二烯单元的泛醌(Q-9)。