Qu Lingyun, Lai Qiliang, Zhu Fengling, Hong Xuguang, Zhang Jinxing, Shao Zongze, Sun Xiuqin
First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1612-1616. doi: 10.1099/ijs.0.026344-0. Epub 2010 Aug 6.
Two novel gram-negative, oxidase- and catalase-positive, rod-shaped bacterial strains, designated YCSA28(T) and YCSA39, were isolated from sediment of Daqiao saltern, Jimo, Qingdao, on the east coast of China. The two strains grew optimally at 28-30 °C, at pH 7.5 and in the presence of 7-8 % (w/v) NaCl. They were assigned to the genus Halomonas, class Gammaproteobacteria, based on 16S rRNA gene sequence analysis. The major cellular fatty acids of the two strains were C(18 : 1)ω7c (42.9 %), C(16 : 0) (23.1 %) and C(16 : 1)ω7c/ω6c (18.0 %), and Q-9 was the major ubiquinone. The G+C content of the DNA of strains YCSA28(T) and YCSA39 was 63.7 and 63.9 mol%, respectively. The predominant respiratory lipoquinone, cellular fatty acid profiles and DNA G+C content of strains YCSA28(T) and YCSA39 were consistent with those of recognized species of the genus Halomonas. Levels of DNA-DNA relatedness between strains YCSA28(T) and YCSA39, between YCSA28(T) and Halomonas ventosae Al12(T), and between YCSA39 and H. ventosae Al12(T) were 95, 45 and 50 %, respectively. Together, these data indicated that strains YCSA28(T) and YCSA39 represent a single novel species of the genus Halomonas, for which the name Halomonas daqiaonensis sp. nov. is proposed. The type strain is YCSA28(T) ( = CGMCC 1.9150(T) = NCCB 100305(T) = MCCC 1B00920(T)).
从中国东海岸青岛即墨大桥盐场的沉积物中分离出两株新型革兰氏阴性、氧化酶和过氧化氢酶阳性的杆状细菌菌株，分别命名为YCSA28(T)和YCSA39。这两株菌株在28-30°C、pH 7.5以及7-8%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列分析，它们被归入γ-变形菌纲嗜盐单胞菌属。这两株菌株的主要细胞脂肪酸为C(18:1)ω7c（42.9%）、C(16:0)（23.1%）和C(16:1)ω7c/ω6c（18.0%），主要泛醌为Q-9。菌株YCSA28(T)和YCSA39的DNA G+C含量分别为63.7 mol%和63.9 mol%。菌株YCSA28(T)和YCSA39的主要呼吸性脂醌、细胞脂肪酸谱以及DNA G+C含量与嗜盐单胞菌属已确认的物种一致。菌株YCSA28(T)和YCSA39之间、YCSA28(T)和通风嗜盐单胞菌Al12(T)之间以及YCSA39和通风嗜盐单胞菌Al12(T)之间的DNA-DNA相关性水平分别为95%、45%和50%。这些数据共同表明，菌株YCSA28(T)和YCSA39代表嗜盐单胞菌属的一个新物种，为此提出新名称大桥嗜盐单胞菌（Halomonas daqiaonensis sp. nov.）。模式菌株为YCSA28(T)（=CGMCC 1.9150(T)=NCCB 100305(T)=MCCC 1B00920(T)）。
