Bartosik-Psujek H, Belniak E, Mitosek-Szewczyk K, Dobosz B, Stelmasiak Z
Department of Neurology, Medical University, Lublin, Poland.
Acta Neurol Scand. 2004 Jun;109(6):390-2. doi: 10.1111/j.1600-0404.2004.00259.x.
Chemokines are involved in the pathogenesis of multiple sclerosis. The aim of the study was to evaluate the effects of immunosuppressive therapy on production of two proinflammatory chemokines--interleukin-8 (IL-8) and RANTES (regulated on activation, normal T cell expressed and secreted).
Twenty-five patients with relapsing-remitting multiple sclerosis were treated with 2-chlorodeoxyadenosine (Cladribine), administered subcutaneously in 6 cycles repeated every 5 weeks. IL-8 and RANTES levels were measured by the enzyme-linked immunoassay (ELISA) method in serum and cerebrospinal fluid (CSF) before and after treatment.
After Cladribine treatment the levels of IL-8 decreased significantly in CSF only, whereas the RANTES levels decreased significantly both in CSF and serum.
Our results suggest that Cladribine therapy might modify the circulating level of RANTES.
趋化因子参与多发性硬化的发病机制。本研究的目的是评估免疫抑制疗法对两种促炎趋化因子——白细胞介素-8（IL-8）和调节激活正常T细胞表达和分泌因子（RANTES）产生的影响。
25例复发缓解型多发性硬化患者接受2-氯脱氧腺苷（克拉屈滨）治疗，皮下注射，每5周重复6个周期。采用酶联免疫吸附测定（ELISA）法在治疗前后检测血清和脑脊液（CSF）中IL-8和RANTES水平。
克拉屈滨治疗后，仅脑脊液中IL-8水平显著下降，而脑脊液和血清中RANTES水平均显著下降。
我们的结果表明，克拉屈滨治疗可能会改变RANTES的循环水平。
