Kikuchi de Beltrán K, Koshikawa N, Saigusa T, Watanabe K, Koshida Y, Kobayashi M
Department of Pharmacology, Nihon University School of Dentistry, Tokyo, Japan.
Eur J Pharmacol. 1992 Apr 22;214(2-3):181-9. doi: 10.1016/0014-2999(92)90117-m.
The role of striatal dopaminergic/cholinergic interactions in the regulation of oral behaviour in rats was studied using methods which resolve distinct patterns of jaw movements, allowing a more accurate quantitative and qualitative analysis. Both dopamine and acetylcholine receptor agonists given either systemically or into the ventral striatum induced repetitive oral movements. However, the cholinergic movements differed from dopaminergic movements as to pattern of activity. Oral movements induced by apomorphine (0.2 mg/kg i.v.) were potentiated by carbachol (0.1 microgram/0.2 microliters) injected into the dorsal striatum, while inhibition was observed when carbachol was injected into the ventral striatum. Pilocarpine (4 mg/kg)-induced oral movements were reduced by injecting flupentixol (10 micrograms/0.2 microliters), but not a combination of SKF 38393 (3 micrograms)+quinpirole (10 micrograms/0.2 microliter), into either the dorsal or the ventral striatum. Oral movements induced by the injection of carbachol (1 microgram/0.2 microliter) into the ventral striatum were enhanced by previous injection of this combination of dopamine receptor agonists into the same site and were inhibited by flupentixol. These results suggest that cholinergic and dopaminergic oral movements are separate behaviors and that the striatal dopamine/acetylcholine interaction in their regulation is neither simply antagonistic or synergistic, nor reciprocal.
利用能够分辨不同下颌运动模式的方法，对纹状体多巴胺能/胆碱能相互作用在大鼠口腔行为调节中的作用进行了研究，从而实现更准确的定量和定性分析。全身给药或向腹侧纹状体注射多巴胺和乙酰胆碱受体激动剂均可诱发重复性口腔运动。然而，胆碱能运动与多巴胺能运动在活动模式上有所不同。静脉注射阿扑吗啡（0.2mg/kg）诱发的口腔运动，在向背侧纹状体注射卡巴胆碱（0.1μg/0.2μl）后增强，而向腹侧纹状体注射卡巴胆碱时则观察到抑制作用。向背侧或腹侧纹状体注射氟哌噻吨（10μg/0.2μl）可减少毛果芸香碱（4mg/kg）诱发的口腔运动，但注射SKF 38393（3μg）+喹吡罗（10μg/0.2μl）的组合则无此效果。向腹侧纹状体注射卡巴胆碱（1μg/0.2μl）诱发的口腔运动，在之前向同一部位注射该多巴胺受体激动剂组合后增强，而被氟哌噻吨抑制。这些结果表明，胆碱能和多巴胺能口腔运动是不同的行为，并且纹状体多巴胺/乙酰胆碱在其调节中的相互作用既不是简单的拮抗或协同作用，也不是相互作用。
