Fujita Satoshi, Adachi Kazunori, Lee Jun, Uchida Takuya, Koshikawa Noriaki, Cools Alexander R
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, 1-8-13, Kanda-Surugadai, Tokyo 101-8310, Chiyoda, Japan.
Eur J Pharmacol. 2004 Jan 19;484(1):75-82. doi: 10.1016/j.ejphar.2003.10.050.
Dopamine and acetylcholine receptor functions in spontaneously hypertensive rats (SHR) and in control progenitor Wistar-Kyoto (WKY) rats were assessed, using dopamine D1-like/D2-like receptor-mediated and acetylcholine receptor-mediated jaw movements as readout parameters. Spontaneous behaviours such as locomotor activity, vacuous chewing, grooming, sniffing and rearing occurred significantly more in SHR than in WKY rats. In the anaesthetised rats, a mixture of SKF 38393 (5 micrograms), a dopamine D1-like receptor agonist, and quinpirole (10 micrograms), a dopamine D2-like receptor agonist, readily produced repetitive jaw movements in WKY rats, but not SHR, when bilaterally injected into the ventrolateral striatum; such injections into the nucleus accumbens shell were ineffective in each strain. Bilateral injections of carbachol (2.5 micrograms each side), an acetylcholine receptor agonist, into the ventrolateral striatum elicited repetitive jaw movements in both SHR and WKY rats, but to a far less degree in SHR. The present study demonstrates that spontaneous behaviours are enhanced in SHR, and that postsynaptic dopamine D1-like/D2-like receptors and acetylcholine receptors in the ventrolateral striatum of SHR are hyposensitive when compared to those of WKY rats.
利用多巴胺D1样/D2样受体介导的和乙酰胆碱受体介导的下颌运动作为读出参数，评估了自发性高血压大鼠（SHR）和对照亲代Wistar-Kyoto（WKY）大鼠的多巴胺和乙酰胆碱受体功能。SHR的自发行为，如运动活动、空嚼、梳理、嗅探和竖毛，比WKY大鼠明显更多。在麻醉大鼠中，当双侧注射到腹外侧纹状体时，多巴胺D1样受体激动剂SKF 38393（5微克）和多巴胺D2样受体激动剂喹吡罗（10微克）的混合物很容易在WKY大鼠中产生重复性下颌运动，但在SHR中则不然；将其注射到伏隔核壳中对每个品系均无效。双侧向腹外侧纹状体注射乙酰胆碱受体激动剂卡巴胆碱（每侧2.5微克）在SHR和WKY大鼠中均引起重复性下颌运动，但在SHR中程度要小得多。本研究表明，SHR的自发行为增强，并且与WKY大鼠相比，SHR腹外侧纹状体中的突触后多巴胺D1样/D2样受体和乙酰胆碱受体反应低下。
