Lux M F, Drake H L
Department of Medical Technology, University of Southern Mississippi, Hattiesburg.
FEMS Microbiol Lett. 1992 Aug 1;74(1):49-56. doi: 10.1016/0378-1097(92)90735-7.
Both Clostridium formicoaceticum and Clostridium aceticum grew chemolithoautotrophically on carbon monoxide plus CO2 in defined medium in the absence of carbohydrates, amino acids, or other carbon and energy sources. Formate supported the growth of both organisms as well in both defined and undefined media (both of which also contained CO2). Hydrogen was stimulatory to the growth of C. formicoaceticum upon first transfer into H2-enriched formate medium; however, neither chemolithoautotrophic growth at the expense of H2 plus CO2 nor hydrogenase could be demonstrated with this acetogen. Consistent with recent findings with other acetogens, numerous aromatic compounds were utilized by C. aceticum and C. formicoaceticum: (i) aromatic methoxyl groups were O-demethylated; (ii) aromatic acrylates were reduced; and (iii) aromatic aldehydes were oxidized. These findings demonstrate that the metabolic potentials of these two acetogens are greater than previously recognized.
甲酸乙酸梭菌和乙酸梭菌在不含碳水化合物、氨基酸或其他碳源和能源的特定培养基中，能以一氧化碳和二氧化碳为原料进行化能自养生长。在特定和非特定培养基（两者均含有二氧化碳）中，甲酸盐也能支持这两种微生物的生长。首次转移到富含氢气的甲酸盐培养基中时，氢气对甲酸乙酸梭菌的生长有促进作用；然而，这种产乙酸菌既不能以氢气和二氧化碳为原料进行化能自养生长，也未检测到氢化酶。与最近对其他产乙酸菌的研究结果一致，乙酸梭菌和甲酸乙酸梭菌能利用多种芳香族化合物：（i）芳香甲氧基发生O-去甲基化；（ii）芳香丙烯酸酯被还原；（iii）芳香醛被氧化。这些发现表明，这两种产乙酸菌的代谢潜能比之前认为的更大。
