Xia Yong-Qing, Guo Tian-Ying, Zhao Hai-Li, Song Mou-Dao, Zhang Bang-Hua, Zhang Bao-Long
Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
J Biomed Mater Res A. 2009 Aug;90(2):326-32. doi: 10.1002/jbm.a.32084.
A novel molecular imprinting method was used to prepare twice-coated silica particles with specific recognition sites for hemoglobin. Chitosan was used as an intermedium to be coated on silica particles via phase inversion process, and the abundance of exposed amine groups (NH2) were active sites for introducing aldehyde groups. After hemoglobin was covalently immobilized by forming imine bonds with the aldehyde groups, acrylamide was then polymerized onto chitosan-coated silica particles to form the recognition sites. The obtained hemoglobin imprinted [molecularly imprinted polymer (MIP)] beads were characterized by FTIR spectroscopy, thermogravimetric analysis (TG), and scanning electron microscopy (SEM). The MIP particles exhibited selectively adsorption for the imprinted protein compared to the nonselectively adsorption for most of proteins of the nonimprinted (NIP) beads.
一种新型分子印迹方法被用于制备对血红蛋白具有特异性识别位点的双层包覆二氧化硅颗粒。壳聚糖用作介质，通过相转化过程包覆在二氧化硅颗粒上，大量暴露的胺基（NH2）是引入醛基的活性位点。血红蛋白通过与醛基形成亚胺键共价固定后，丙烯酰胺再聚合到壳聚糖包覆的二氧化硅颗粒上以形成识别位点。所获得的血红蛋白印迹[分子印迹聚合物（MIP）]微球通过傅里叶变换红外光谱（FTIR）、热重分析（TG）和扫描电子显微镜（SEM）进行表征。与非印迹（NIP）微球对大多数蛋白质的非选择性吸附相比，MIP颗粒对印迹蛋白表现出选择性吸附。
