[茶多酚和茶色素对HepG2细胞端粒酶活性的影响]

[Effects of tea polyphenols and tea pigments on telomerase activity of HepG2 cells].

作者信息

Jia Xu-dong, Han Chi, Chen Jun-shi

机构信息

Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2004 May;38(3):159-61.

PMID:15182480

Abstract

OBJECTIVE

This study is to investigate the effect of tea polyphenols and tea pigments on telomerase activity of human liver cancer cell line, HepG2 cells.

METHODS

TRAP-PCR-ELISA was applied to investigate the telomerase activity.

RESULTS

Telomerase was positive in tea polyphenols treated groups, tea pigments treated groups and blank control group. Telomerase activities (A(450 approximately 690) values) were 1.56 and 1.46 in 50 mg/L and 100 mg/L tea polyphenols-treated groups, 1.55 and 1.49 in 50 mg/L and 100 mg/L tea pigments-treated groups, respectively. The results showed that telomerase activity was significantly inhibited by tea polyphenols and tea pigments treatment as compared with the blank control group (A(450 approximately 690) = 2.11).

CONCLUSIONS

Tea polyphenols and tea pigments could significantly inhibit telomerase activity of HepG2 cells, and telomerase activity may be a useful biomarker for cancer chemoprevention.

摘要

目的

本研究旨在探讨茶多酚和茶色素对人肝癌细胞系HepG2细胞端粒酶活性的影响。

方法

应用端粒重复序列扩增-聚合酶链反应-酶联免疫吸附测定法（TRAP-PCR-ELISA）检测端粒酶活性。

结果

茶多酚处理组、茶色素处理组及空白对照组端粒酶均呈阳性。50mg/L和100mg/L茶多酚处理组的端粒酶活性（A(450约690)值）分别为1.56和1.46，50mg/L和100mg/L茶色素处理组分别为1.55和1.49。结果显示，与空白对照组（A(450约690)=2.11）相比，茶多酚和茶色素处理可显著抑制端粒酶活性。