[茶多酚和茶色素对人肝癌细胞株HepG2细胞凋亡的影响]
[Effects of tea polyphenols and tea pigments on apoptosis in HepG2 cell line].
作者信息
Jia Xu-Dong, Han Chi, Chen Jun-Shi
机构信息
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
出版信息
Wei Sheng Yan Jiu. 2005 Jan;34(1):73-5.
OBJECTIVE
To investigate the effect of tea polyphenols and tea pigments on apoptosis in HepG2 cells.
METHODS
HepG2 cells were seeded at a density of 5 x 10(5)/well in six-well culture dishes. The cells were then treated with 50 or 100 mg/L tea pigments and harvested at 48h by trypsinization. Agrose electrophoresis was applied to investigate DNA-LADDER, Bcl-2 and Bax protein expression were detected by Western blot.
RESULTS
Tea polyphenols and tea pigments induced the appearance of DNA-LADDER; Western blot analysis demonstrated that Bcl-2 expression was significantly inhibited and the expression of Bax was significantly induced by tea polyphenols and tea pigments.
CONCLUSION
Induction of apoptosis may be an important mechanism of cancer chemoprevention by tea.
目的
研究茶多酚和茶色素对HepG2细胞凋亡的影响。
方法
将HepG2细胞以5×10(5)/孔的密度接种于六孔培养板中。然后用50或100mg/L茶色素处理细胞，48小时后用胰蛋白酶消化收集细胞。采用琼脂糖电泳检测DNA梯状条带，用蛋白质免疫印迹法检测Bcl-2和Bax蛋白表达。
结果
茶多酚和茶色素诱导出现DNA梯状条带；蛋白质免疫印迹分析表明，茶多酚和茶色素显著抑制Bcl-2表达，显著诱导Bax表达。
结论
诱导细胞凋亡可能是茶化学预防癌症的重要机制。
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