印度北部土壤和水样中分枝杆菌分离程序的优化
Optimization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India.
作者信息
Parashar Deepti, Chauhan D S, Sharma V D, Chauhan Aradhana, Chauhan S V S, Katoch V M
机构信息
Central JALMA Institute for Leprosy and Other Mycobacterial Diseases, Indian Council of Medical Research, Tajganj, Agra 28201, India.
出版信息
Appl Environ Microbiol. 2004 Jun;70(6):3751-3. doi: 10.1128/AEM.70.6.3751-3753.2004.
Abstract
For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.
摘要
为了分离环境中的分枝杆菌，已制定了一种去污程序，即先用3%十二烷基硫酸钠加4%氢氧化钠处理（快速生长菌和缓慢生长菌分别处理15分钟和30分钟），然后用2%溴化十六烷基三甲铵孵育（快速生长和缓慢生长的分枝杆菌分别孵育5分钟和15分钟）；该程序被发现能完全消除其他微生物的污染，仅分离出分枝杆菌。
相似文献
1
Optimization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India.
Appl Environ Microbiol. 2004 Jun;70(6):3751-3. doi: 10.1128/AEM.70.6.3751-3753.2004.
2
Evaluation of BacT/Alert 3D liquid culture system for recovery of mycobacteria from clinical specimens using sodium dodecyl (lauryl) sulfate-NaOH decontamination.
J Clin Microbiol. 2001 Oct;39(10):3799-800. doi: 10.1128/JCM.39.10.3799-3800.2001.
3
Recovery of Non-tuberculous Mycobacteria from Water is Influenced by Phenotypic Characteristics and Decontamination Methods.
Curr Microbiol. 2020 Apr;77(4):621-631. doi: 10.1007/s00284-019-01704-w. Epub 2019 May 20.
4
Isolation of Nontuberculous Mycobacteria from the Environment of Ghanian Communities Where Buruli Ulcer Is Endemic.
Appl Environ Microbiol. 2016 Jun 30;82(14):4320-4329. doi: 10.1128/AEM.01002-16. Print 2016 Jul 15.
5
[Identification and isolation of non-tuberculous mycobacteria from environmental samples].
Mikrobiyol Bul. 2010 Jul;44(3):395-403.
6
Isolation of mycobacteria from undecontaminated specimens with selective 7H10 medium.
J Clin Microbiol. 1981 Jan;13(1):76-9. doi: 10.1128/jcm.13.1.76-79.1981.
7
Comparison of some decontamination methods and growth media for isolation of mycobacteria from northern brook waters.
J Appl Microbiol. 1997 Jan;82(1):121-7. doi: 10.1111/j.1365-2672.1997.tb03306.x.
8
9
[Atypical mycobacteria isolated from soil and water].
Ann Sclavo. 1982 Sep-Oct;24(5):496-502.
10
Decontamination of an Extracorporeal Membrane Oxygenator Contaminated With Mycobacterium chimaera.
Infect Control Hosp Epidemiol. 2017 Oct;38(10):1244-1246. doi: 10.1017/ice.2017.163. Epub 2017 Aug 14.
引用本文的文献
1
Polyphasic taxonomic description of sp. nov. and sp. nov. and their biotechnological potential.
Int J Syst Evol Microbiol. 2025 Mar;75(3). doi: 10.1099/ijsem.0.006716.
2
Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid-fluorescence in situ hybridization.
J Fish Dis. 2023 Jan;46(1):47-59. doi: 10.1111/jfd.13716. Epub 2022 Sep 21.
3
A New Culture Method for the Detection of Non-Tuberculous Mycobacteria in Water Samples from Heater-Cooler Units and Extracorporeal Membrane Oxygenation Machines.
Int J Environ Res Public Health. 2022 Aug 26;19(17):10645. doi: 10.3390/ijerph191710645.
4
Characterization of novel hydrocarbon-degrading Gordonia paraffinivorans and Gordonia sihwensis strains isolated from composting.
PLoS One. 2019 Apr 18;14(4):e0215396. doi: 10.1371/journal.pone.0215396. eCollection 2019.
5
Isolation of nontuberculous mycobacteria from soil using Middlebrook 7H10 agar with increased malachite green concentration.
AMB Express. 2017 Dec;7(1):69. doi: 10.1186/s13568-017-0373-6. Epub 2017 Mar 23.
6
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U.
BMC Microbiol. 2016 Jun 17;16(1):111. doi: 10.1186/s12866-016-0734-3.
7
Prevalence of Non-Tuberculous Mycobacteria in Hospital Waters of Major Cities of Khuzestan Province, Iran.
Front Cell Infect Microbiol. 2016 Apr 13;6:42. doi: 10.3389/fcimb.2016.00042. eCollection 2016.
本文引用的文献
1
Evaluation of procedures for isolation of nontuberculous mycobacteria from soil and water.
Appl Environ Microbiol. 1994 Mar;60(3):1021-4. doi: 10.1128/aem.60.3.1021-1024.1994.
2
Effect of PANTA on growth of Mycobacterium kansasii in BACTEC 12B medium.
J Clin Microbiol. 1995 Aug;33(8):2012-5. doi: 10.1128/jcm.33.8.2012-2015.1995.
3
Epidemiology of infection by nontuberculous mycobacteria. I. Geographic distribution in the eastern United States.
Am Rev Respir Dis. 1980 Jun;121(6):931-7. doi: 10.1164/arrd.1980.121.6.931.
4
The occurrence of Mycobacterium kansasii in tapwater.
Tubercle. 1980 Mar;61(1):21-6. doi: 10.1016/0041-3879(80)90055-0.
5
How environmental mycobacteria may predetermine the protective efficacy of BCG.
Tubercle. 1981 Mar;62(1):55-62. doi: 10.1016/0041-3879(81)90037-4.
6
Methods for selective isolation of mycobacteria from the environment.
Can J Microbiol. 1981 Jan;27(1):1-7. doi: 10.1139/m81-001.
7
A sputum swab culture method for tubercle bacilli using cetrimide compared with two other swab culture methods and the concentration culture method.
Tubercle. 1969 Sep;50(3):299-304. doi: 10.1016/0041-3879(69)90055-5.
8
Examination of soil in the brisbane area for organisms of the Mycobacterium avium-intracellulare-scrofulaceum complex.
Pathology. 1974 Jul;6(3):269-73. doi: 10.3109/00313027409068997.
9
Selective isolation of mycobacteria from soil: a statistical analysis approach.
J Gen Microbiol. 1988 Mar;134(3):849-55. doi: 10.1099/00221287-134-3-849.
10
Diagnosis and treatment of disease caused by nontuberculous mycobacteria.
Am Rev Respir Dis. 1990 Oct;142(4):940-53. doi: 10.1164/ajrccm/142.4.940.
Zotero 插件