Polyphasic taxonomic description of sp. nov. and sp. nov. and their biotechnological potential.

strains DSM 116494 and DSM 116496 were isolated from sediment samples of the River Oker in Braunschweig, Germany, and subjected to a polyphasic taxonomic study and genome mining for specialized secondary metabolites. Phenotypic, genetic and genomic data confirmed the assignment of these strains to the genus. Pairwise 16S rRNA gene sequence similarity values between the strains and validly named species reached 99.5 and 99.7% for strains DSM 116494 and DSM 116496, respectively. Genome-based phylogeny demonstrated that and species were the close relatives to strain DSM 116494, while species was the nearest neighbour to strain DSM 116496. Digital DNA-DNA hybridization and average nucleotide identity comparisons of the genomic sequence of the strains and their close phylogenomic relatives revealed that values were below the determined threshold of 70 and 95-96% for prokaryotic species demarcation, respectively. The strains were distinguished from their close neighbours based on biochemical, chemotaxonomic and enzymatic data. Given these results, the strains merit being affiliated to novel species within the genus , for which the names sp. nov. (=OG2.3=DSM 116494=KCTC 59408) and sp. nov. (=OG3.14=DSM 116496=KCTC 59410) are proposed. Strains DSM 116494 and DSM 116496 harboured several biosynthetic gene clusters encoding potentially novel antimicrobial and anticancer compounds. Crude extracts of strains DSM 116494 and DSM 116496 inhibited the growth of Gram-negative bacteria ( ΔtolC, ) and a multi-drug-resistant Gram-positive, .