Cano Josep, Guarro Josep, Gené Josepa
Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201-Reus, Tarragona, Spain.
J Clin Microbiol. 2004 Jun;42(6):2450-4. doi: 10.1128/JCM.42.6.2450-2454.2004.
Colletotrichum species have caused human infections in recent years. Because of the difficulties in recognizing them in vitro, we have designed a quick and unambiguous molecular test, based on the amplification of a specific fragment of the internal transcribed spacer 1 region, to distinguish any Colletotrichum isolate from other fungi, including the common pathogenic species. Analysis of the sequences of the ribosomal DNA (rDNA) fragment showed sufficient variability to clearly separate the five species of Colletotrichum that are of clinical interest, i.e., Colletotrichum coccodes, C. crassipes, C. dematium, C. gloeosporioides, and C. graminicola. Sequencing of the D1-D2 region of the large-subunit rDNA gene also supported these results. Additionally, we reviewed the most suitable morphological characteristics for the in vitro identification of these increasingly important opportunistic fungi.
近年来,炭疽菌属已导致人类感染。由于在体外识别它们存在困难,我们设计了一种快速且明确的分子检测方法,基于对内部转录间隔区1区域特定片段的扩增,以区分任何炭疽菌分离株与其他真菌,包括常见的致病物种。对核糖体DNA(rDNA)片段序列的分析显示出足够的变异性,能够清晰地分离出具有临床意义的五种炭疽菌属物种,即球炭疽菌、粗柄炭疽菌、黑炭疽菌、胶孢炭疽菌和禾生炭疽菌。大亚基rDNA基因D1-D2区域的测序也支持了这些结果。此外,我们还回顾了用于体外鉴定这些日益重要的机会性真菌的最合适形态特征。