Bragança Carlos A D, Damm Ulrike, Baroncelli Riccardo, Massola Júnior Nelson S, Crous Pedro W
Universidade Federal do Recôncavo da Bahia, 710, CEP 44380-000, Cruz das Almas-BA, Brazil.
CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; Senckenberg Museum of Natural History, Görlitz PF 300 154, 02806 Görlitz, Germany.
Fungal Biol. 2016 Apr;120(4):547-561. doi: 10.1016/j.funbio.2016.01.011. Epub 2016 Feb 2.
Although Colletotrichum acutatum was recently investigated and shown to be a species complex comprising about 30 species, the name is still used in its broad sense for anthracnose pathogens of fruits in Brazil. In this study, a multilocus molecular analysis was carried out based on a dataset of ITS, HIS3, GAPDH, CHS-1, TUB2 and ACT sequences of Colletotrichum strains belonging to the C. acutatum species complex from fruits collected in different regions in Brazil combined with sequences of ex-type and other reference strains of species belonging to this complex. The strains were revealed to belong to Colletotrichum nymphaeae, Colletotrichum melonis, Colletotrichum abscissum and one new species, namely Colletotrichum paranaense, from apple and peach. Morphological descriptions of the new species and a strain closely related to but diverging from C. melonis are provided. From the data presently available, the most common species on apple fruits in Brazil is C. nymphaeae. In a pathogenicity test, strains of all four species caused lesions on detached apple, peach and guava fruits, except for strain CBS 134730 that did not infect guava fruits.
尽管尖孢炭疽菌（Colletotrichum acutatum）最近经过调查，显示为一个包含约30个物种的复合种，但在巴西，该名称仍被广泛用于指代果实炭疽病的病原菌。在本研究中，基于来自巴西不同地区果实上的尖孢炭疽菌复合种的胶孢炭疽菌（C. acutatum）菌株的ITS、HIS3、GAPDH、CHS-1、TUB2和ACT序列数据集，结合该复合种的模式菌株和其他参考菌株的序列，进行了多位点分子分析。结果显示，这些菌株属于睡莲炭疽菌（Colletotrichum nymphaeae）、甜瓜炭疽菌（Colletotrichum melonis）、离层炭疽菌（Colletotrichum abscissum）以及一个新物种，即来自苹果和桃子的巴拉那炭疽菌（Colletotrichum paranaense）。文中提供了新物种以及与甜瓜炭疽菌亲缘关系较近但又有所差异的一个菌株的形态学描述。根据目前可得的数据，巴西苹果果实上最常见的物种是睡莲炭疽菌。在致病性测试中，除了CBS 134730菌株未感染番石榴果实外，所有四个物种的菌株均在离体苹果、桃子和番石榴果实上造成了病斑。
