通过黄酮类化合物含量对佐尔菲诺地方品种（菜豆）进行种质特性鉴定。

Germplasm characterization of Zolfino landraces (Phaseolus vulgaris L.) by flavonoid content.

作者信息

Romani Annalisa, Vignolini Pamela, Galardi Carlotta, Mulinacci Nadia, Benedettelli Stefano, Heimler Daniela

机构信息

Dipartimento di Scienze Farmaceutiche, Università degli Studi di Firenze, via U. Schiff 6, 50019 Sesto Fiorentino, Italy.

出版信息

J Agric Food Chem. 2004 Jun 16;52(12):3838-42. doi: 10.1021/jf0307402.

DOI:10.1021/jf0307402

PMID:15186105

Abstract

The flavonoid composition of three phenotypes of "Zolfino" landraces, a typical bean grown in Tuscany, has been elucidated by means of HPLC-DAD and HPLC-MS analysis. Flavonols, isoflavones, and anthocyanins have been separated and determined in the different samples chosen on the basis of their seed coat color. A flavonol that has not been previously found in Phaseolus vulgaris L. seeds has been characterized. The quantitative data show the presence of flavonols (ranging from 709 to 118 mg/kg of fresh weight), isoflavones (ranging from 14 to 2 mg/kg of fresh weight), and anthocyanins, in black beans only. These results show that this genotype could be very interesting from a nutritional point of view.

摘要

通过高效液相色谱-二极管阵列检测法（HPLC-DAD）和高效液相色谱-质谱分析法（HPLC-MS），已阐明了“佐尔菲诺”地方品种三种表型的类黄酮组成，“佐尔菲诺”是托斯卡纳种植的一种典型豆类。根据种皮颜色选择不同样品，对黄酮醇、异黄酮和花青素进行了分离和测定。已鉴定出一种此前未在菜豆种子中发现的黄酮醇。定量数据表明，仅黑豆中存在黄酮醇（鲜重范围为709至118毫克/千克）、异黄酮（鲜重范围为14至2毫克/千克）和花青素。这些结果表明，从营养角度来看，这种基因型可能非常有趣。