Aparicio-Fernandez Xochitl, Yousef Gad G, Loarca-Pina Guadalupe, de Mejia Elvira, Lila Mary Ann
Programa de Posgrado en Alimentos del Centro de la Republica (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autonoma de Queretaro, Queretaro, 76010, Mexico.
J Agric Food Chem. 2005 Jun 1;53(11):4615-22. doi: 10.1021/jf047802o.
The common bean contains phytochemicals, including phenolic compounds, which can provide health benefits to the consumer. Our objective was to characterize the polyphenolic compounds present in the seed coat of Black Jamapa bean and to test fractionation methods that permit the recovery of polyphenolics in their naturally occurring forms. A 100% methanol extract from seed coats was subjected to different chromatographic fractionation methods. Analysis by HPLC-MS revealed that a better separation of phytochemicals was achieved using direct silica gel fractionation, which allowed more accurate identification of compounds, especially of the flavonols. Anthocyanins, flavanol monomers, and heterogeneous flavanol oligomers up to hexamers were detected. To our knowledge, this is the first time that myricetin glycoside and proanthocyanidin oligomers containing (epi)-gallocatechin have been reported in the black bean. The fractionation methods used in this study produced large quantities of natural mixtures of flavonoids suitable for testing bioactivity and phytochemical interactions.
普通菜豆含有包括酚类化合物在内的植物化学物质，这些物质能为消费者带来健康益处。我们的目标是鉴定黑哈马帕豆种皮中存在的多酚化合物，并测试能以天然存在形式回收多酚类物质的分馏方法。对种皮的100%甲醇提取物进行了不同的色谱分馏方法。通过HPLC-MS分析发现，使用直接硅胶分馏能更好地分离植物化学物质，这使得对化合物，尤其是黄酮醇的鉴定更加准确。检测到了花青素、黄烷醇单体以及直至六聚体的异质黄烷醇低聚物。据我们所知，这是首次在黑豆中报道杨梅素糖苷和含有（表）儿茶素的原花青素低聚物。本研究中使用的分馏方法产生了大量适合测试生物活性和植物化学相互作用的天然类黄酮混合物。
