Zhang Yan, Qian Yun, Feng Ting, Cai Lingbo, Liu Jiayin
Human Clinical Reproductive Medicine Center, Department of Obstetrics & Gynecology, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Zhonghua Nan Ke Xue. 2004 May;10(5):348-50.
To separate high-quality sperm by PureSperm centrifugation applied to intrauterine insemination (IUI) cycles.
We compared the separate results after washing the semen with one-layer and two-layer PureSperm gradient centrifugation methods and two-layer Percoll gradient centrifugation method, and used the recovered high-quality sperm for IUI.
The density of the sperm washed with one-layer PureSperm centrifugation method was significantly higher than that washed with two-layer Percoll and two-layer PureSperm centrifugation methods(P < 0.01), but there were no differences in all the results between the use of two-layer Percoll and two-layer PureSperm(P > 0.05). No significant differences in the motility, teratozoospermia and IUI results were found when the three methods were used for sperm preparation(P > 0.05). The percentage of morphologically normal sperm was markedly increased, and the non-sperm components such as leucocytes, epithelial cells and cellular fragments were significantly reduced after washed by the two methods.
PureSperm centrifugation is a safe, efficient and easy method for separating high-quality sperm on intrauterine insemination cycles.
应用PureSperm离心法分离高质量精子用于宫腔内人工授精（IUI）周期。
我们比较了用单层和双层PureSperm梯度离心法以及双层Percoll梯度离心法洗涤精液后的分离结果，并将回收的高质量精子用于IUI。
单层PureSperm离心法洗涤后的精子密度显著高于双层Percoll和双层PureSperm离心法洗涤后的精子密度（P<0.01），但双层Percoll和双层PureSperm的所有结果之间无差异（P>0.05）。三种方法用于精子制备时，在活力、畸形精子症和IUI结果方面未发现显著差异（P>0.05）。经两种方法洗涤后，形态正常精子的百分比显著增加，白细胞、上皮细胞和细胞碎片等非精子成分显著减少。
PureSperm离心法是一种在宫腔内人工授精周期中分离高质量精子的安全、高效且简便的方法。
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