Huang H Y, Lai Y M, Lee C L, Chang M Y, Chang S Y, Soong Y K
Department of Obstetrics & Gynecology, Chang Gung Memorial Hospital, Taipei, Taiwan, R.O.C.
Changgeng Yi Xue Za Zhi. 1996 Mar;19(1):24-9.
The purpose of this study was to compare the different sperm manipulation methods on the recovery rate of motile sperm that was collected to use in the intrauterine insemination. Three hundred and thirty-five IUI (intrauterine insemination) cycle from a total of 963 treatment cycles was performed for non-peritoneal factor infertility couples. The sperm separation methods were swim-up, swim-down and Percoll gradient (since Mar. 1992). We compared the different sperm preparation methods in normal and abnormal semen analysis group. Our results show that in the abnormal semen samples (oligospermic approximately, asthenospermic approximately), the Percoll gradient method is superior to the swim-up and swim-down methods on the recovery of a higher number of total, motile and active motile spermatozoa (p < 0.05), even though the sperm quality before preparation was rather poorer than the samples prepared by the other two methods. The pregnancy rate in both groups are not statistically significant. We conclude that the Percoll gradient is an improved method for semen manipulation as it allows greater recovery of sperm with higher motility in intrauterine insemination.
本研究的目的是比较不同的精子处理方法对用于宫内授精的活动精子回收率的影响。对963个治疗周期中的335个宫内授精（IUI）周期的非腹膜因素不孕夫妇进行了研究。精子分离方法为上游法、下游法和Percoll梯度法（自1992年3月起）。我们比较了正常和异常精液分析组中不同的精子制备方法。我们的结果表明，在异常精液样本（大致为少精子症、大致为弱精子症）中，Percoll梯度法在回收更多的总精子、活动精子和活动力强的精子方面优于上游法和下游法（p<0.05），尽管制备前的精子质量比其他两种方法制备的样本差得多。两组的妊娠率无统计学差异。我们得出结论，Percoll梯度法是一种改进的精液处理方法，因为它能在宫内授精中使具有更高活力的精子回收率更高。
