Rebmann V, Doxiadis I, Kubens B S, Grosse-Wilde H
Department of Immunology, University Hospital, Essen, FRG.
Vox Sang. 1992;62(2):117-23. doi: 10.1111/j.1423-0410.1992.tb01182.x.
We determined the C4 plasma concentrations of 48 genotypically CA4- and C4B-defined unrelated individuals from 34 families with a total of 196 members by an enzyme-linked immunosorbent assay using Rg:1,2 (C4A) and Ch:1 (C4B) specific monoclonal antibodies. The results obtained allowed the establishment of rules for the detection of C4 Q0 alleles in the heterozygous form and of C4A gene duplications. In the present study seven homoduplications of the C4A 3 allotype were defined which had not been detected by allotyping. This procedure allows the simple, reliable, and quick determination of Rg:1,2 and Ch:1 plasma levels which are not influenced by daily rhythms of C4 production.
我们通过使用Rg:1,2（C4A）和Ch:1（C4B）特异性单克隆抗体的酶联免疫吸附测定法，测定了来自34个家庭、共196名成员的48名基因分型为CA4 - 和C4B定义的无关个体的C4血浆浓度。所得结果有助于建立检测杂合形式的C4 Q0等位基因和C4A基因重复的规则。在本研究中，定义了7种C4A 3同种异型的同型重复，这些重复在同种异型分型中未被检测到。该方法可简单、可靠且快速地测定不受C4产生的日常节律影响的Rg:1,2和Ch:1血浆水平。
