da Silva Marcelo Barreto Spillere, Farges Roseli Coimbra, Fröde Tânia Silvia
Department of Clinical Analysis, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Universitário, Florianópolis, SC, Brazil.
Mediators Inflamm. 2004 Apr;13(2):93-103. doi: 10.1080/09629350410001688486.
Studies on peripheral benzodiazepine receptor function have yielded a diverse list of activities of which the anti-inflammatory effects need to be further examined.
To evaluate the role of steroids, nitric oxide and adenosine-deaminase in the anti-inflammatory effect of PK11195.
Pleurisy was induced by intrapleural injection of carrageenan in mice pre-treated or not with PK11195. Leukocytes, exudation, adenosine-deaminase (ADA) activity and nitric oxide (NO) level were measured. Steroid involvement was evaluated by pre-treatment with D,L-aminogluthetimide before PK11195.
Leukocytes, exudation and NO levels were reduced by PK11195 in the early (4 h) phase. In the late (48 h) phase, PK11195 decreased leukocytes and ADA activity. D,L-aminogluthetimide reversed the effect of PK11195 on exudate (4 h), as well as total and differential leukocytes and NO levels (48 h).
Steroids, NO and ADA are implicated in the anti-inflammatory action of PK11195.
关于外周苯二氮䓬受体功能的研究产生了一系列不同的活性,其中抗炎作用有待进一步研究。
评估类固醇、一氧化氮和腺苷脱氨酶在PK11195抗炎作用中的作用。
通过向经PK11195预处理或未预处理的小鼠胸膜腔内注射角叉菜胶诱导胸膜炎。测量白细胞、渗出液、腺苷脱氨酶(ADA)活性和一氧化氮(NO)水平。在PK11195之前用D,L-氨基谷氨酰胺预处理来评估类固醇的参与情况。
PK11195在早期(4小时)阶段降低了白细胞、渗出液和NO水平。在晚期(48小时)阶段,PK11195降低了白细胞和ADA活性。D,L-氨基谷氨酰胺逆转了PK11195对渗出液(4小时)以及总白细胞和分类白细胞及NO水平(48小时)的影响。
类固醇、NO和ADA参与了PK11195的抗炎作用。