随机抽取的北非牛奶和奶酪样本中黄曲霉毒素M1的出现情况。
Occurrence of aflatoxin M1 in randomly selected North African milk and cheese samples.
作者信息
Elgerbi A M, Aidoo K E, Candlish A A G, Tester R F
机构信息
Food Research Laboratory, School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, G4 OBA, UK.
出版信息
Food Addit Contam. 2004 Jun;21(6):592-7. doi: 10.1080/02652030410001687690.
Forty-nine samples of raw cow's milk and 20 samples of fresh white soft cheese were collected directly from 20 local dairy factories in the north-west of Libya and analysed for the presence of aflatoxin M1 (AFM1). The samples were analysed using a high-performance liquid chromatography technique for toxin detection and quantification. Thirty-five of the 49 milk samples (71.4%) showed AFM1 levels between 0.03 and 3.13 ng ml(-1) milk. Multiple analyses of five milk samples free of AFM1 artificially contaminated with concentrations of AFM1 at 0.01, 0.05, 0.1, 1.0 and 3.0 ng ml(-1) showed average recoveries of 66.85, 72.41, 83.29, 97.94 and 98.25%, with coefficients of variations of 3.77, 4.11, 1.57, 1.29 and 0.54%, respectively. Fifteen of 20 white soft cheese samples (75.0%) showed the presence of AFM1 in concentrations between 0. 11 and 0.52 ng g(-1) of cheese. Multiple assays of five cheese samples free of AFM1 spiked with different concentration of AFM1 (0.1, 0.5, 1.0 and 3.0 ng g(-1)) showed average recoveries of 63.23, 78.14,83.29 and 88.68%, with coefficients of variation of 1.53, 9.90, 4.87 and 3.79%, respectively. The concentrations of AFM1 were lower in the cheese products than in the raw milk samples.
从利比亚西北部的20家当地乳制品厂直接采集了49份生牛奶样本和20份新鲜白软奶酪样本，分析其中黄曲霉毒素M1（AFM1）的存在情况。使用高效液相色谱技术对样本进行毒素检测和定量分析。49份牛奶样本中有35份（71.4%）的AFM1含量在0.03至3.13 ng/ml牛奶之间。对5份不含AFM1的牛奶样本进行人工污染，使其AFM1浓度分别为0.01、0.05、0.1、1.0和3.0 ng/ml，多次分析显示平均回收率分别为66.85%、72.41%、83.29%、97.94%和98.25%，变异系数分别为3.77%、4.11%、1.57%、1.29%和0.54%。20份白软奶酪样本中有15份（75.0%）检测出AFM1，浓度在0.11至0.52 ng/g奶酪之间。对5份不含AFM1的奶酪样本添加不同浓度的AFM1（0.1、0.5、1.0和3.0 ng/g）进行多次检测，平均回收率分别为63.23%、78.14%、83.29%和88.%，变异系数分别为1.53%、9.90%、4.87%和3.79%。奶酪产品中AFM1的浓度低于生牛奶样本。
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