采用液相色谱-串联质谱法测定意大利普通人群尿液中的苯乙烯代谢物。

Determination of urinary styrene metabolites in the general Italian population by liquid chromatography-tandem mass spectrometry.

作者信息

Manini Paola, De Palma Giuseppe, Andreoli Roberta, Goldoni Matteo, Mutti Antonio

机构信息

Laboratory of Industrial Toxicology, Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma, via Gramsci 14, 43100 Parma, Italy.

出版信息

Int Arch Occup Environ Health. 2004 Aug;77(6):433-6. doi: 10.1007/s00420-004-0526-1. Epub 2004 Jun 15.

DOI:10.1007/s00420-004-0526-1

PMID:15205964

Abstract

OBJECTIVE

To apply mass spectrometry-based techniques for the determination of background levels of metabolites of widespread organic solvents, such as styrene, in unexposed subjects from the general population.

METHODS

The study population consisted of 129 healthy Italian subjects, not occupationally exposed to styrene (67 men; 37% smokers). Urinary levels of mandelic acid (MA) and phenylglyoxylic acid (PGA) were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

RESULTS

Using LC-MS/MS, we were able to detect the peaks of MA and PGA in 100% of samples. The distribution of styrene metabolites was log-normal, the geometric means of MA and PGA concentrations being 0.443 mg/g creatinine [geometric standard deviation (GSD) 2.34] and 0.107 mg/g creatinine (GSD 3.49), respectively. The reference intervals estimated for MA and PGA were 0.084-2.339 and 0.009-1.238 mg/g creatinine, respectively.

CONCLUSION

The application of highly sensitive and selective mass spectrometry-based analytical techniques could be useful in the definition of reference values for metabolites of organic pollutants and for better assessment of low-level occupational exposure to organic solvents.

摘要

目的

应用基于质谱的技术测定普通人群中未接触过的个体（如苯乙烯）等广泛使用的有机溶剂代谢物的背景水平。

方法

研究人群包括129名健康的意大利受试者，他们未职业性接触苯乙烯（67名男性；37%为吸烟者）。通过液相色谱 - 串联质谱法（LC-MS/MS）测定尿中扁桃酸（MA）和苯乙酮酸（PGA）的水平。

结果

使用LC-MS/MS，我们能够在100%的样本中检测到MA和PGA的峰。苯乙烯代谢物的分布呈对数正态分布，MA和PGA浓度的几何平均值分别为0.443 mg/g肌酐[几何标准差（GSD）2.34]和0.107 mg/g肌酐（GSD 3.49）。MA和PGA估计的参考区间分别为0.084 - 2.339和0.009 - 1.238 mg/g肌酐。