Irmak Hasan, Buzgan Turan, Evirgen Omer, Akdeniz Hayrettin, Demiroz A Pekcan, Abdoel Theresia H, Smits Henk L
Department of Infectious Diseases and Clinical Microbiology, Yüzüncü Yil University, Van, Turkey.
Am J Trop Med Hyg. 2004 Jun;70(6):688-94.
The clinical utility of two complementary tests for brucellosis, the Brucella IgM and IgG flow assays, was evaluated in a hospital in eastern Turkey. The results show that the flow assays are convenient diagnostic tests for use in endemic areas. A positive result in the flow assays was obtained in 91% and 97% of the admission sera from adult and pediatric patients with brucellosis, respectively, and the sensitivity at admission was 100% for culture-confirmed brucellosis. The assay system performed equally well in diagnosing patients at different stages of illness including patients with acute, subacute, or chronic disease and with relapse. The results of the flow assays correlated well with those of a serum agglutination test at a cut-off > or =1:160. The agreement was 92%. Application of the flow assays on serum samples collected during a village survey for brucellosis after an outbreak demonstrated their diagnostic potential as field tests.
在土耳其东部的一家医院对两种用于布鲁氏菌病的互补检测方法——布鲁氏菌IgM和IgG流式检测法的临床效用进行了评估。结果表明，流式检测法是适用于流行地区的便捷诊断检测方法。在成年和儿科布鲁氏菌病患者的入院血清中，分别有91%和97%的样本通过流式检测法得到阳性结果，对于经培养确诊的布鲁氏菌病，入院时的敏感性为100%。该检测系统在诊断疾病不同阶段的患者时表现同样出色，包括急性、亚急性或慢性疾病患者以及复发患者。当血清凝集试验的截断值≥1:160时，流式检测法的结果与之相关性良好。一致性为92%。在一次布鲁氏菌病爆发后的村庄调查中，将流式检测法应用于采集的血清样本，证明了其作为现场检测方法的诊断潜力。
