Wang J, Meyering-Vos M, Hoffmann K H
Department of Animal Ecology I, University of Bayreuth, 95440 Bayreuth, Germany.
Mol Cell Endocrinol. 2004 Nov 30;227(1-2):41-51. doi: 10.1016/j.mce.2004.07.014.
Cricket- or B-type allatostatins [W(X(6))W-amides] inhibit the biosynthesis of juvenile hormones in vitro in crickets. Peptides of this family are present also in other insects where they may bare different functions. Here we report the identification of a partial sequence of the B-type preproallatostatin from Gryllus bimaculatus. By PCR screening of a random primer cDNA library and by RACE, a 535bp 3'cDNA sequence was obtained which encodes a putative translation product of 85 amino acids, containing three copies of Grybi-AST B1 and one copy each of Grybi-AST B2, Grybi-AST B3, and Grybi-AST B6. The last represents a novel member of this peptide family. By means of one-step RT-PCR, RNA dot blot, and RT in situ PCR analyses the mRNA expression of the gene in the central nervous system and the digestive tract of female adult crickets was demonstrated. The results confirm that the B-type allatostatins of G. bimaculatus are brain-gut peptides.
蟋蟀型或B型咽侧体抑制素[W(X(6))W酰胺]在体外可抑制蟋蟀体内保幼激素的生物合成。该家族的肽类在其他昆虫中也存在，在这些昆虫中它们可能具有不同的功能。在此，我们报告了双斑蟋B型前咽侧体抑制素部分序列的鉴定。通过对随机引物cDNA文库的PCR筛选和RACE技术，获得了一个535bp的3' cDNA序列，该序列编码一个推定的由85个氨基酸组成的翻译产物，包含三个Grybi-AST B1拷贝、一个Grybi-AST B2拷贝、一个Grybi-AST B3拷贝和一个Grybi-AST B6拷贝。最后一个代表该肽家族的一个新成员。通过一步法RT-PCR、RNA斑点印迹和RT原位PCR分析，证实了该基因在成年雌性蟋蟀中枢神经系统和消化道中的mRNA表达。结果证实，双斑蟋的B型咽侧体抑制素是脑肠肽。
