Gong Jüfang, Shi Feng, Shao Linxiang, Zheng Xiaohua
College of Chemistry and Life Sciences Zhejiang Normal University, Jinhua 321004, China.
Wei Sheng Yan Jiu. 2004 May;33(3):324-7.
To study the effects of soybean phospholipids on learning and memory abilities and contents of protein and lipids in brain of mice.
Mice were randomly divided into four groups: low dose (2.5 g/kg), middle dose(5.0 g/kg) and high dose (10.0 g/kg) of soybean Phospholipids in diet and control group (distilled water). The mice were fed with soybean phospholipids everyday while control groups were fed with distilled water for 30 days. After learning and memory abilities were measured, the mice were killed and contents of protein, fatty acids and phospholipids in brain of mice were determined.
The learning and memory abilities of mice fed with middle and high dose of soybean Phospholipids were improved obviously(P < 0.05), while memory abilities of low dose's mice were stronger than that of control(P < 0.05). (2) The brain weight v.8 body weight of those in three doses' group was obviously increased, and the protein content in brain was significantly higher compared with control(P < 0.05). (3) The contents of linoleic acid(C18: 2), linolenic acid(C18: 3) and ratio of polyunsaturated fatty acids(PUFA) to total fatty acids (TFA) obviously increased (P < 0.05). In brain of high dose mice, there was obvious change in the content of linolenic acid(C18: 3) in middle's (P < 0.05). (4) The contents of phosphatidylcholine (PS) phosphatidylethanolamine (PE), Phosphatidylcholine (PC) and phospholipids (PL) obviously increase (P < 0.05), while ratio of SM to PC was decreased significantly in brain of high dose mice(P < 0.05). The contents of PS, PC and PL were increased obviously(P < 0.05).
Soybean phospholipids may increase contents of protein, PUFA and PL in brain of mice and improve learning and memory abilities of mice effectively in a dose-response manner.
研究大豆磷脂对小鼠学习记忆能力及脑内蛋白质和脂质含量的影响。
将小鼠随机分为四组：饮食中添加低剂量（2.5 g/kg）、中剂量（5.0 g/kg）和高剂量（10.0 g/kg）大豆磷脂组以及对照组（蒸馏水）。每天给小鼠喂食大豆磷脂，对照组喂食蒸馏水，持续30天。在测量学习记忆能力后，处死小鼠并测定小鼠脑内蛋白质、脂肪酸和磷脂的含量。
（1）中、高剂量大豆磷脂喂养的小鼠学习记忆能力明显提高（P<0.05），低剂量组小鼠的记忆能力强于对照组（P<0.05）。（2）三个剂量组小鼠的脑重与体重之比明显增加，脑内蛋白质含量显著高于对照组（P<0.05）。（3）亚油酸（C18:2）、亚麻酸（C18:3）含量及多不饱和脂肪酸（PUFA）与总脂肪酸（TFA）的比值明显升高（P<0.05）。高剂量组小鼠脑内亚麻酸（C18:3）含量与中剂量组相比有明显变化（P<0.05）。（4）脑内磷脂酰丝氨酸（PS）、磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）及总磷脂（PL）含量明显增加（P<0.05），高剂量组小鼠脑内鞘磷脂与磷脂酰胆碱的比值显著降低（P<0.05）。PS、PC和PL含量明显增加（P<0.05）。
大豆磷脂可增加小鼠脑内蛋白质、PUFA和PL的含量，并以剂量反应方式有效提高小鼠的学习记忆能力。
