Gay Mélanie, Berthe Franck C J, Le Roux Frédérique
Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (IFREMER), 17390 La Tremblade, France.
Dis Aquat Organ. 2004 Apr 21;59(1):49-56. doi: 10.3354/dao059049.
In an attempt to develop a reproducible experimental model of bacterial infection in Crassostrea gigas, oysters taken from very localised sub-populations suffering natural mortality outbreaks were used in cohabitation trials under laboratory conditions. From these trials, a collection of Vibrio strains was isolated from moribund and healthy oysters. In a second step, strains were experimentally tested for virulence by means of injection into healthy oysters. This screening revealed a span of virulence among isolated strains from none to medium. When pooling injected strains, results suggest increased virulence. Vibrio strains may have additive/synergistic action leading to higher C. gigas mortality rates in experimental challenges. Although the study initially aimed to develop a simple experimental model, a complex of interactions emerged between several bacterial strains during the pathogenic process in their molluscan host. Selected strains provide a suitable model of experimental disease for further studies and better understanding of bacterial interaction and pathogenesis in C. gigas.
为了建立一个可重复的太平洋牡蛎细菌感染实验模型，从遭受自然死亡爆发的非常局部的亚种群中采集的牡蛎被用于实验室条件下的同居试验。从这些试验中，从濒死和健康的牡蛎中分离出了一批弧菌菌株。在第二步中，通过将菌株注射到健康牡蛎中对其毒力进行了实验测试。这种筛选揭示了分离菌株之间的毒力范围，从无到中等。当将注射的菌株合并时，结果表明毒力增加。弧菌菌株可能具有相加/协同作用，导致在实验挑战中太平洋牡蛎的死亡率更高。尽管该研究最初旨在建立一个简单的实验模型，但在其软体动物宿主的致病过程中，几种细菌菌株之间出现了复杂的相互作用。所选菌株为进一步研究以及更好地理解太平洋牡蛎中的细菌相互作用和发病机制提供了一个合适的实验性疾病模型。
