Simi S, Musio A, Vatteroni L, Piras A, Rainaldi G
Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.
Cancer Genet Cytogenet. 1992 Aug;62(1):81-7. doi: 10.1016/0165-4608(92)90044-9.
Cytogenetic changes were investigated during the spontaneous progression of CHEF18 Chinese hamster cells towards tumorigenicity. We further report the chromosomal characterization of a series of spontaneous anchorage-independent clones, as well as of a series of tumor-derived cell lines resulting from injection of late passage cells in nude mice. The high karyotypic homogeneity (presence of four marker chromosomes strictly associated in all the metaphases analyzed) in all clones and tumor-derived cell lines prompted us to alter the specific pattern of chromosomal aberrations in order to identify which if any of the aberrations were more strictly related to transformation. For this purpose we treated a tumor-derived cell line with Colcemid and analyzed the reversion of anchorage-independent phenotype in the subclones showing an altered association of the four marker chromosomes. We conclude that two of four marker chromosomes contribute to anchorage independence.
在CHEF18中国仓鼠细胞自发向致瘤性发展的过程中，对细胞遗传学变化进行了研究。我们进一步报告了一系列自发的非贴壁依赖性克隆以及一系列由晚期传代细胞注射到裸鼠体内产生的肿瘤衍生细胞系的染色体特征。所有克隆和肿瘤衍生细胞系中高度的核型同质性（在所有分析的中期相中均存在四条紧密相关的标记染色体）促使我们改变染色体畸变的特定模式，以确定哪些畸变与转化更为密切相关。为此，我们用秋水仙酰胺处理了一个肿瘤衍生细胞系，并分析了在四条标记染色体关联发生改变的亚克隆中非贴壁依赖性表型的逆转情况。我们得出结论，四条标记染色体中的两条对非贴壁依赖性起作用。
