Canepa Gaspar E, Silber Ariel M, Bouvier León A, Pereira Claudio A
Laboratorio de Biología Molecular de Trypanosoma cruzi, Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Argentina.
FEMS Microbiol Lett. 2004 Jul 1;236(1):79-84. doi: 10.1016/j.femsle.2004.05.021.
Trypanosoma cruzi, the etiological agent of Chagas disease, uses arginine for several metabolic processes, including energy reserves management. In the present work, a novel low-affinity arginine transport system has been studied. Maximum velocity (97 pmol min(-1) per 10(7) cells), and an estimate for the apparent Km value (350 microM) of this arginine transporter, were 6-fold and 80-fold higher respectively, when compared with the previously described high-affinity arginine transport system. This transport activity seems to be H+ -mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle.
克氏锥虫是恰加斯病的病原体，它利用精氨酸进行多种代谢过程，包括能量储备管理。在本研究中，对一种新型低亲和力精氨酸转运系统进行了研究。与先前描述的高亲和力精氨酸转运系统相比，该精氨酸转运体的最大速度（每10⁷个细胞97 pmol min⁻¹）和表观Km值估计（350 μM）分别高出6倍和80倍。这种转运活性似乎是由H⁺介导的，对其他氨基酸如蛋氨酸具有广泛的特异性，并且在寄生虫的生长曲线和生命周期中受到调节。
