Fundación Instituto Leloir, Universidad de Buenos Aires and CONICET, Argentina.
FEMS Microbiol Lett. 2010 May;306(2):97-102. doi: 10.1111/j.1574-6968.2010.01936.x. Epub 2010 Feb 24.
Trypanosoma cruzi, the aetiological agent of Chagas' disease, is exposed to extremely different environment conditions during its life cycle, and transporters are key molecules for its adaptive regulation. Amino acids, and particularly arginine, are essential components in T. cruzi metabolism. In this work, a novel T. cruzi arginine permease was identified by screening different members of the AAAP family (amino acid/auxin permeases) in yeast complementation assays using a toxic arginine analogue. One gene candidate, TcAAAP411, was characterized as a very specific, high-affinity, l-arginine permease. This work is the first identification of the molecular components involved specifically in amino acid transport in T. cruzi and provides new insights for further validation of the TcAAAP family as functional permeases.
克氏锥虫，恰加斯病的病原体，在其生命周期中会暴露在极其不同的环境条件下，而转运蛋白是其适应调节的关键分子。氨基酸，特别是精氨酸，是克氏锥虫代谢的必需成分。在这项工作中，通过使用有毒精氨酸类似物在酵母互补测定中筛选不同的 AAAP 家族（氨基酸/生长素转运蛋白）成员，鉴定了一种新型克氏锥虫精氨酸转运蛋白。一个候选基因，TcAAAP411，被表征为一种非常特异、高亲和力的 l-精氨酸转运蛋白。这项工作首次鉴定了参与克氏锥虫氨基酸转运的分子成分，并为进一步验证 TcAAAP 家族作为功能转运蛋白提供了新的见解。
