Ahn Cheol-Hee, Chae Su Young, Bae You Han, Kim Sung Wan
School of Materials Science and Engineering, Hyperstructured Organic Materials Research Center, Seoul National University, Seoul 151-742, South Korea.
J Control Release. 2004 Jul 7;97(3):567-74. doi: 10.1016/j.jconrel.2004.04.002.
Biodegradable and non-toxic multi-block copolymers based on poly(L-lysine) and poly(ethylene glycol) were synthesized. Synthesized copolymers showed almost negligible cytotoxicity above 95% cell viability and transfection efficiency compared to the PLL homopolymer with molecular weight of 25,700. Biodegradation under physiological conditions revealed that the molecular weight of copolymers decreased to 20% of the initial molecular weight within 72 h. Transfection efficiencies of copolymers were not affected by the presence of serum, while that of PLL homopolymer decreased to the level of naked DNA in the presence of serum. Based on the results, the new copolymers are believed to be a potentially efficient carrier for the delivery of bioactive agents.
合成了基于聚（L-赖氨酸）和聚（乙二醇）的可生物降解且无毒的多嵌段共聚物。与分子量为25700的聚-L-赖氨酸均聚物相比，合成的共聚物在细胞活力高于95%时显示出几乎可忽略不计的细胞毒性和转染效率。生理条件下的生物降解表明，共聚物的分子量在72小时内降至初始分子量的20%。共聚物的转染效率不受血清存在的影响，而聚-L-赖氨酸均聚物在血清存在下的转染效率降至裸DNA的水平。基于这些结果，新型共聚物被认为是生物活性剂递送的潜在有效载体。
