Plenderleith M B, Wright L L, Snow P J
Department of Anatomy, University of Queensland, Brisbane, Australia.
Brain Res Dev Brain Res. 1992 Jul 24;68(1):103-9. doi: 10.1016/0165-3806(92)90252-r.
The plant lectin Bandeiraea simplicifolia I-B4 binds to the soma and central terminals of a subpopulation of unmyelinated primary sensory neurones in the adult rat. The binding site of this lectin is thought to be the terminal alpha-D-galactose residue of a membrane associated glycoconjugate which may be involved in the development of specific connections between small diameter primary sensory neurones and second order neurones in the superficial dorsal horn of the spinal cord. To begin to investigate this possibility we have examined the development of lectin binding in the dorsal horn of pre- and postnatal rats. Lectin binding first appeared on axon profiles in the superficial dorsal horn of the spinal cord at embryonic days 18/19. Previous studies in the rat have revealed that the central processes of small diameter primary sensory neurones enter the dorsal horn at embryonic days 18/19. Our findings suggest that the glycoconjugate to which this lectin binds, is expressed by the central processes of small diameter primary sensory neurones as they grow into the spinal cord. It is therefore possible that this glycoconjugate is involved in the development of topographically ordered neural connections within the dorsal horn of the spinal cord.
植物凝集素单叶豆素I-B4可与成年大鼠无髓初级感觉神经元亚群的胞体和中枢终末结合。这种凝集素的结合位点被认为是膜相关糖缀合物的末端α-D-半乳糖残基，它可能参与脊髓背角浅层中小直径初级感觉神经元与二级神经元之间特定连接的形成。为了开始研究这种可能性，我们检查了产前和产后大鼠背角中凝集素结合的发育情况。凝集素结合最早在胚胎第18/19天出现在脊髓背角浅层的轴突轮廓上。此前在大鼠中的研究表明，小直径初级感觉神经元的中枢突在胚胎第18/19天进入背角。我们的研究结果表明，这种凝集素结合的糖缀合物在小直径初级感觉神经元的中枢突生长进入脊髓时表达。因此，这种糖缀合物有可能参与脊髓背角内拓扑有序神经连接的形成。
