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1H, 13C and 15N backbone resonance assignments for TEM-1, a 28.9 kDa beta-lactamase from E. coli.

作者信息

Savard Pierre-Yves, Sosa-Peinado Alejandro, Levesque Roger C, Makinen Marvin W, Gagné Stéphane M

出版信息

J Biomol NMR. 2004 Jul;29(3):433-4. doi: 10.1023/B:JNMR.0000032503.96942.68.

DOI:10.1023/B:JNMR.0000032503.96942.68
PMID:15213455
Abstract
摘要

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引用本文的文献

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The Structural Dynamics of Engineered β-Lactamases Vary Broadly on Three Timescales yet Sustain Native Function.工程β-内酰胺酶的结构动力学在三个时间尺度上变化很大,但维持天然功能。
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2
15N, 13C and 1H backbone resonance assignments of an artificially engineered TEM-1/PSE-4 class A β-lactamase chimera and its deconvoluted mutant.人工构建的TEM-1/PSE-4 A类β-内酰胺酶嵌合体及其去卷积突变体的15N、13C和1H主链共振归属
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3
Chimeric β-lactamases: global conservation of parental function and fast time-scale dynamics with increased slow motions.

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NMR View: A computer program for the visualization and analysis of NMR data.NMR 视图:用于可视化和分析 NMR 数据的计算机程序。
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An ultrahigh resolution structure of TEM-1 beta-lactamase suggests a role for Glu166 as the general base in acylation.
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Overexpression and biosynthetic deuterium enrichment of TEM-1 beta-lactamase for structural characterization by magnetic resonance methods.
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Molecular basis of antibiotic resistance and beta-lactamase inhibition by mechanism-based inactivators: perspectives and future directions.基于机制的失活剂对抗生素耐药性和β-内酰胺酶抑制的分子基础:观点与未来方向
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The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data.13C化学位移指数:一种利用13C化学位移数据鉴定蛋白质二级结构的简单方法。
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Molecular structure of the acyl-enzyme intermediate in beta-lactam hydrolysis at 1.7 A resolution.β-内酰胺水解过程中酰基酶中间体的分子结构,分辨率为1.7埃。
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