Achkar Jacqueline M, Burda Sherri T, Konings Frank A J, Urbanski Mateusz M, Williams Constance A U, Seifen Dorothee, Kahirimbanyi Martha N, Vogler Mary, Parta Mark, Lupatkin Helene C, Zolla-Pazner Susan, Nyambi Phillipe N
Department of Medicine, New York University School of Medicine, NY 10010, USA.
J Acquir Immune Defic Syndr. 2004 Jul 1;36(3):835-44. doi: 10.1097/00126334-200407010-00011.
To document infection with HIV type 1 (HIV-1) group M non-B subtypes in individuals living in New York City.
From October 1999 through April 2003, HIV-1-seropositive individuals were selected from 3 clinics in New York City based on having risk factors for infection with HIV-1 non-B subtypes.
HIV-1 RNA was extracted from plasma samples, and partial gag, pol, or env genes were amplified by PCR analysis. The infecting HIV-1 group M subtype was determined based on results of either heteroduplex mobility assay or sequencing and phylogenetic analysis.
Ninety-seven subjects were enrolled in the study. Of the 97 subjects, 91 (94%) were selected based on having emigrated from a non-European country, while 6 (6%) were native United States citizens. Subtypes were successfully determined in 53 (55%) of the 97 plasma samples tested. The subtypes in 2 plasma samples were unclassifiable. HIV-1 infections were classified as those due to the following group M subtypes: A (n = 4; 7%), B (n = 12; 22%), C (n = 8; 15%), F (n = 2; 4%), CRF01_AE-like (n = 7; 13%), CRF02_AG-like (n = 19; 34%), an intersubtype recombinant form G/A (n = 1; 2%), and unclassifiable viruses (n = 2; 4%).
This study reveals infection with a broad variety of HIV-1 group M subtypes mostly in the immigrant population of New York City as well as how several non-B subtypes are being introduced into the United States.
记录纽约市居民感染1型人类免疫缺陷病毒（HIV-1）M组非B亚型的情况。
从1999年10月至2003年4月，根据具有感染HIV-1非B亚型的危险因素，从纽约市的3家诊所选取HIV-1血清反应阳性个体。
从血浆样本中提取HIV-1 RNA，通过聚合酶链反应（PCR）分析扩增部分gag、pol或env基因。根据异源双链迁移率分析或测序及系统发育分析结果确定感染的HIV-1 M组亚型。
97名受试者纳入研究。97名受试者中，91名（94%）因从非欧洲国家移民而入选，6名（6%）为美国本土公民。在检测的97份血浆样本中，53份（55%）成功确定了亚型。2份血浆样本中的亚型无法分类。HIV-1感染分类如下：A（n = 4；7%）、B（n = 12；22%）、C（n = 8；15%）、F（n = 2；4%）、CRF01_AE样（n = 7；13%）、CRF02_AG样（n = 19；34%）、G/A亚型重组形式（n = 1；2%）以及无法分类的病毒（n = 2；4%）。
本研究揭示了纽约市移民人群中感染多种HIV-1 M组亚型的情况，以及几种非B亚型如何传入美国。
