Seidel-Morgenstern Andreas
Institut für Verfahrenstechnik, Otto-von-Guericke-Universität, Magdeburg D-39106, Germany.
J Chromatogr A. 2004 May 28;1037(1-2):255-72. doi: 10.1016/j.chroma.2003.11.108.
In order to design and to optimise preparative liquid chromatography, the knowledge of the underlying thermodynamic functions, i.e. the adsorption isotherms, is of large importance. Usually these functions can not be predicted and various techniques have been suggested to determine them experimentally. In this paper, several important methods to measure adsorption equilibrium data are discussed and evaluated. The main focus is set on dynamic methods analysing concentration profiles that could be detected at the outlet of fixed-beds packed with the stationary phase of interest. The theoretical background of the different methods is explained using classical equilibrium theory and the equilibrium dispersion model. Each method is illustrated based on experimental data collected in our laboratory. Based on these personal experiences recommendations are given regarding the potential and the applicability of the methods discussed.
为了设计和优化制备液相色谱，了解其潜在的热力学函数，即吸附等温线，至关重要。通常这些函数无法预测，人们已提出各种技术来通过实验确定它们。本文讨论并评估了几种测量吸附平衡数据的重要方法。主要重点放在分析在填充有所关注固定相的固定床出口处可检测到的浓度分布的动态方法上。使用经典平衡理论和平衡扩散模型解释了不同方法的理论背景。基于我们实验室收集的实验数据对每种方法进行了说明。基于这些个人经验，针对所讨论方法的潜力和适用性给出了建议。
